Chemical Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Inorg Chem. 2009 Dec 7;48(23):10868-70. doi: 10.1021/ic9013703.
Although widely used in bioassays, the spectrofluorimetric method described here uses the antenna effect as a tool to probe the thermodynamic parameters of ligands that sensitize lanthanide luminescence. The Eu(3+) coordination chemistry, solution thermodynamic stability, and photophysical properties of the spermine-based hydroxypyridonate octadentate chelator 3,4,3-LI(1,2-HOPO) are reported. The complex Eu(III)(3,4,3-LI(1,2-HOPO)) luminesces with a long lifetime (805 mus) and a quantum yield of 7.0% in aqueous solution, at pH 7.4. These remarkable optical properties were exploited to determine the high (and proton-independent) stability of the complex (log beta(110) = 20.2(2)) and to define the influence of the ligand scaffold on the stability and photophysical properties.
尽管在生物测定中广泛应用，但这里描述的荧光光谱法使用天线效应作为工具来探测敏化镧系元素发光的配体的热力学参数。报道了基于亚精胺的羟基吡啶二酮八齿螯合剂 3,4,3-LI(1,2-HOPO)的 Eu(3+)配位化学、溶液热力学稳定性和光物理性质。配合物 Eu(III)(3,4,3-LI(1,2-HOPO))在 pH 7.4 的水溶液中具有长寿命（805 mus）和 7.0%的量子产率的发光，这些显著的光学性质被用来确定配合物的高（与质子无关）稳定性（log beta(110) = 20.2(2))，并定义配体支架对稳定性和光物理性质的影响。
