Department of Electronics and Electrical Engineering, The Bioelectronics Research Centre, University of Glasgow, Glasgow, UK G12 8LT.
Anal Chem. 2009 Dec 1;81(23):9828-33. doi: 10.1021/ac902010s.
Lab-on-a-chip technologies have the potential to deliver significant technological advances in modern biomedicine, through the ability to provide appropriate low-cost microenvironments for screening cells. However, to date, few studies have investigated the suitability of poly(dimethylsiloxane) (PDMS) for live cell culture. Here, we describe an inexpensive method for production of reusable, optical-grade PDMS microculture chips which provide a static and self-contained microwell system analogous to conventional polystyrene multiwell plates. We use these structures to probe the effects of PDMS upon live cell culture bioassays, using time-lapse fluorescence imaging to explore the toxicity of the substrate. We use three model systems to explore the efficacy of the microstructured devices: (i) live cell culture, (ii) adenoviral gene delivery to mammalian cells, and (iii) gravity enforced formation of multicellular tumor spheroids (MCTS). Results show that PDMS is nontoxic to cells, as their viability and growth characteristic in PDMS-based platforms is comparable to that of their polystyrene counterparts.
微流控芯片技术有可能通过为细胞筛选提供适当的低成本微环境，在现代生物医学领域带来重大的技术进步。然而，迄今为止，很少有研究探讨聚二甲基硅氧烷（PDMS）是否适用于活细胞培养。在这里，我们描述了一种生产可重复使用的、光学级 PDMS 微培养芯片的廉价方法，该芯片提供了类似于传统聚苯乙烯多孔板的静态、自包含的微井系统。我们使用这些结构来探测 PDMS 对活细胞培养生物测定的影响，使用延时荧光成像来探索基底的毒性。我们使用三个模型系统来探索微结构器件的功效：（i）活细胞培养，（ii）腺病毒基因转染哺乳动物细胞，和（iii）重力强制形成多细胞肿瘤球体（MCTS）。结果表明，PDMS 对细胞无毒，因为它们在基于 PDMS 的平台上的活力和生长特征与聚苯乙烯对照物相当。
