Hirama Hirotada, Satoh Taku, Sugiura Shinji, Shin Kazumi, Onuki-Nagasaki Reiko, Kanamori Toshiyuki, Inoue Tomoya
Research Center for Ubiquitous MEMS and Micro Engineering, National Institute of Advanced Industrial Science and Technology, 1-2-1 Namiki, Tsukuba, Ibaraki 305-8564, Japan.
Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
J Biosci Bioeng. 2019 May;127(5):641-646. doi: 10.1016/j.jbiosc.2018.10.019. Epub 2018 Nov 23.
The use of organ-on-a-chip (OOC) devices is a promising alternative to existing cell-based assays and animal testing in drug discovery. A rapid prototyping method with polydimethylsiloxane (PDMS) is widely used for developing OOC devices. However, because PDMS tends to absorb small hydrophobic molecules, the loss of test compounds in cell-based assays and increases in background fluorescence during observation often lead to biased results in cell-based assays. To address this issue, we have fabricated a glass-based OOC device and characterized the medium flow and molecular absorption properties in comparison with PDMS-based devices. Consequently, we revealed that the glass device generated a stable medium flow, restricted the absorption of small hydrophobic molecules, and showed enhanced cell adhesiveness. This glass device is expected to be applicable to precise cell-based assays to evaluate small hydrophobic molecules, for which PDMS devices cannot be applied because of their absorption of small hydrophobic molecules.
在药物研发中，使用芯片器官（OOC）设备是现有基于细胞的检测方法和动物试验的一种有前景的替代方案。一种用聚二甲基硅氧烷（PDMS）的快速原型制作方法被广泛用于开发OOC设备。然而，由于PDMS倾向于吸收小的疏水分子，基于细胞的检测中测试化合物的损失以及观察期间背景荧光的增加常常导致基于细胞的检测结果出现偏差。为了解决这个问题，我们制作了一种基于玻璃的OOC设备，并与基于PDMS的设备相比，对培养基流动和分子吸收特性进行了表征。结果，我们发现玻璃设备产生了稳定的培养基流动，限制了小疏水分子的吸收，并表现出增强的细胞粘附性。这种玻璃设备有望适用于精确的基于细胞的检测，以评估小疏水分子，而基于PDMS的设备由于吸收小疏水分子而无法应用于此类检测。
