Department of Microbial Genetics, Institute of Molecular Biology, Slovak Academy of Science, Bratislava, Slovakia.
FEMS Microbiol Lett. 2010 Jan;302(1):58-68. doi: 10.1111/j.1574-6968.2009.01832.x. Epub 2009 Oct 23.
In both rod-shaped Bacillus subtilis and Escherichia coli cells, Min proteins are involved in the regulation of division septa formation. In E. coli, dynamic oscillation of MinCD inhibitory complex and MinE, a topological specificity protein, prevents improper polar septation. However, in B. subtilis no MinE is present and no oscillation of Min proteins can be observed. The function of MinE is substituted by that of an unrelated DivIVA protein, which targets MinCD to division sites and retains them at the cell poles. We inspected cell division when the E. coli Min system was introduced into B. subtilis cells. Expression of these heterologous Min proteins resulted in cell elongation. We demonstrate here that E. coli MinD can partially substitute for the function of its B. subtilis protein counterpart. Moreover, E. coli MinD was observed to have similar helical localization as B. subtilis MinD.
在杆状的枯草芽孢杆菌和大肠杆菌细胞中，Min 蛋白参与调节分裂隔膜的形成。在大肠杆菌中，MinCD 抑制复合物和拓扑特异性蛋白 MinE 的动态振荡防止了不当的极性隔膜形成。然而，在枯草芽孢杆菌中不存在 MinE，也观察不到 Min 蛋白的振荡。MinE 的功能被一个不相关的 DivIVA 蛋白所取代，该蛋白将 MinCD 靶向到分裂位点，并将其保留在细胞两极。我们在将大肠杆菌 Min 系统引入枯草芽孢杆菌细胞时检查了细胞分裂。这些异源 Min 蛋白的表达导致细胞伸长。我们在这里证明，大肠杆菌 MinD 可以部分替代其枯草芽孢杆菌蛋白对应物的功能。此外，观察到大肠杆菌 MinD 具有与枯草芽孢杆菌 MinD 相似的螺旋定位。
