Department of Food Technology, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand.
J Food Prot. 2009 Nov;72(11):2293-300. doi: 10.4315/0362-028x-72.11.2293.
Gelatin electrospun (e-spun) fiber mats containing nisin were produced by electrostatic spinning of gelatin-nisin in 70% (vol/vol) acetic acid aqueous solutions. Varying nisin loading concentration (0 to 3% [wt/wt]) did not affect the fiber average diameter, whereas increasing gelatin concentration from 20 to 24% (wt/vol) caused an increase in the average diameter. All nisin-loaded gelatin e-spun fiber mats demonstrated inhibition against Lactobacillus plantarum TISTR 850. However, all fiber mats were fragile and easily dissolved in water. Cross-linking by saturated glutaraldehyde vapor at 37 degrees C for 5 min was done to strengthen the mat. Tensile strength, Young's modulus, and elongation of the cross-linked gelatin-nisin e-spun fiber mats varied in the range of 2.6 to 20.3 MPa, 163 to 966 MPa, and 1.7 to 5.9% , respectively. Cross-linking did not affect the mat's inhibition activity against L. plantarum TISTR 850. Nisin retention in cross-linked antimicrobial gelatin e-spun fiber mats was in the range of 1.0 to 1.22% . Increasing temperature caused an increase in nisin release, but increasing water activity did not cause a significant difference in nisin release over 50 h. After storage at 25 degrees C for 5 months, the antimicrobial gelatin e-spun fiber mat still showed inhibition against L. plantarum TISTR 850. The mats also inhibited the growth of Staphylococcus aureus and Listeria monocytogenes but not Salmonella Typhimurium.
明胶静电纺丝（e-spun）纤维垫中含有乳链菌肽，通过在 70%（体积/体积）的醋酸水溶液中静电纺丝明胶-乳链菌肽来制备。乳链菌肽负载浓度（0 至 3%[重量/重量]）的变化不会影响纤维的平均直径，而明胶浓度从 20%增加到 24%（重量/体积）会导致平均直径增加。所有负载乳链菌肽的明胶 e-spun 纤维垫均对植物乳杆菌 TISTR 850 表现出抑制作用。然而，所有纤维垫都很脆弱，容易在水中溶解。通过在 37°C 下用饱和戊二醛蒸气交联 5 分钟来增强垫子。交联的明胶-乳链菌肽 e-spun 纤维垫的拉伸强度、杨氏模量和伸长率分别在 2.6 至 20.3 MPa、163 至 966 MPa 和 1.7 至 5.9%的范围内变化。交联不影响垫子对植物乳杆菌 TISTR 850 的抑制活性。交联抗菌明胶 e-spun 纤维垫中的乳链菌肽保留率在 1.0 至 1.22%的范围内。升高温度会导致乳链菌肽释放增加，但在 50 小时内，升高水活度不会导致乳链菌肽释放产生显著差异。在 25°C 下储存 5 个月后，抗菌明胶 e-spun 纤维垫仍对植物乳杆菌 TISTR 850 表现出抑制作用。这些垫子还抑制金黄色葡萄球菌和单核细胞增生李斯特菌的生长，但不抑制肠炎沙门氏菌的生长。
