Department of Surgery, Emory University, Atlanta, GA 30332, USA.
Anal Biochem. 2010 Mar 1;398(1):132-4. doi: 10.1016/j.ab.2009.11.001. Epub 2009 Nov 10.
In this note, we demonstrate the utility of bifunctional fluorescent linkers to facilitate the construction of peptide microarrays with either an N- or a C-terminal alkylamine for directionally preferred peptide immobilization. Significantly, these small tags facilitate high-performance liquid chromatography (HPLC) profiling while limiting interference with antigen-antibody interactions after peptide immobilization. In a model peptide-antibody binding assay, a sequence-dependent orientation effect of antibody binding to a series of peptide ligands was demonstrated. This approach provides a strategy that can be applied to a variety of peptide microarray-based detection systems.
在本说明中，我们展示了双功能荧光接头的实用性，可用于构建具有 N-或 C-末端伯胺的肽微阵列，以实现定向优先肽固定。重要的是，这些小标签在限制肽固定后抗原-抗体相互作用干扰的同时，促进了高效液相色谱（HPLC）分析。在模型肽-抗体结合分析中，证明了抗体与一系列肽配体结合的序列依赖性定向效应。该方法提供了一种可应用于各种基于肽微阵列的检测系统的策略。
