Laboratory of Animal Biotechnology and Genomics, Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV 26506-6108, United States.
J Proteomics. 2010 Feb 10;73(4):778-89. doi: 10.1016/j.jprot.2009.10.014. Epub 2009 Nov 10.
Muscle deterioration arises as a physiological response to elevated energetic demands of fish during sexual maturation and spawning. Previously, we used this model to characterize the transcriptomic mechanisms associated with fish muscle degradation and identified potential biological markers of muscle growth and quality. However, transcriptional measurements do not necessarily reflect changes in active mature proteins. Here we report the characterization of proteomic profile in degenerating muscle of rainbow trout in relation to the female reproductive cycle using a LC/MS-based label-free protein quantification method. A total of 146 significantly changed proteins in atrophying muscles (FDR <5%) was identified. Proteins were clustered according to their gene ontology identifiers. Muscle atrophy was associated with decreased abundance in proteins of anaerobic respiration, protein biosynthesis, monooxygenases, follistatins, and myogenin, as well as growth hormone, interleukin-1 and estrogen receptors. In contrast, proteins of MAPK/ERK kinase, glutamine synthetase, transcription factors, Stat3, JunB, Id2, and NFkappaB inhibitor, were greater in atrophying muscle. These changes are discussed in light of the mammalian muscle atrophy paradigm and proposed fish-specific mechanisms of muscle degradation. These data will help identify genes associated with muscle degeneration and superior flesh quality in rainbow trout, facilitating identification of genetic markers for muscle growth and quality.
肌肉退化是鱼类在性成熟和产卵期间为满足能量需求增加而产生的一种生理反应。此前，我们使用该模型来描述与鱼类肌肉降解相关的转录组机制，并确定了肌肉生长和质量的潜在生物学标志物。然而，转录水平的测量并不一定反映出活跃的成熟蛋白的变化。在这里，我们使用基于 LC/MS 的无标记蛋白定量方法，报道了与雌性生殖周期相关的虹鳟鱼退化肌肉的蛋白质组特征。在萎缩肌肉中鉴定到了 146 个丰度有显著变化的蛋白（FDR<5%）。根据基因本体标识符对蛋白进行聚类。肌肉萎缩与无氧呼吸、蛋白合成、单加氧酶、卵泡抑素和肌生成素以及生长激素、白细胞介素-1 和雌激素受体的丰度降低有关。相反，在萎缩肌肉中，MAPK/ERK 激酶、谷氨酰胺合成酶、转录因子、Stat3、JunB、Id2 和 NFkappaB 抑制剂的蛋白丰度更高。这些变化与哺乳动物肌肉萎缩模型以及鱼类特有的肌肉降解机制进行了讨论。这些数据将有助于确定与虹鳟鱼肌肉退化和优质肉质相关的基因，从而有助于鉴定肌肉生长和质量的遗传标记。
