Key Laboratory of Tropical &Subtropical Fishery Resource Application &Cultivation, Pearl River Fisheries Research Institute of CAFS, Xingyu Road No. 1, Guangzhou 510380, China.
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Xingangxi Road, Guangzhou 510275, China.
Sci Rep. 2017 Apr 3;7:45950. doi: 10.1038/srep45950.
Fish muscle growth is important for the rapidly developing global aquaculture industry, particularly with respect to production and quality. Changes in muscle fibre size are accomplished by altering the balance between protein synthesis and proteolysis. However, our understanding regarding the effects of different protein sources on fish muscle proteins is still limited. Here we report on the proteomic profile of muscle fibre hyperplasia in grass carp fed only with whole faba bean. From the results, a total of 99 significantly changed proteins after muscle hyperplasia increase were identified (p < 0.05, ratio <0.5 or >2). Protein-protein interaction analysis demonstrated the presence of a network containing 56 differentially expressed proteins, and muscle fibre hyperplasia was closely related to a protein-protein network of 12 muscle component proteins. Muscle fibre hyperplasia was also accompanied by decreased abundance in the fatty acid degradation and calcium signalling pathways. In addition, metabolism via the pentose phosphate pathway decreased in grass carp after ingestion of faba bean, leading to haemolysis. These findings could provide a reference for the prevention and treatment of human glucose-6-phosphate dehydrogenase deficiency ("favism").
鱼类肌肉生长对于快速发展的全球水产养殖业至关重要，特别是在产量和质量方面。肌肉纤维大小的变化是通过改变蛋白质合成和蛋白水解之间的平衡来实现的。然而，我们对于不同蛋白质源对鱼类肌肉蛋白质的影响的理解仍然有限。在这里，我们报告了仅用完整的蚕豆喂养的草鱼肌肉纤维增生的蛋白质组学特征。结果表明，肌肉增生增加后共鉴定出 99 种显著变化的蛋白质（p<0.05，比率<0.5 或>2）。蛋白质-蛋白质相互作用分析表明存在一个包含 56 种差异表达蛋白质的网络，肌肉纤维增生与 12 种肌肉成分蛋白质的蛋白质-蛋白质网络密切相关。肌肉纤维增生还伴随着脂肪酸降解和钙信号通路中丰度的降低。此外，蚕豆摄入后，草鱼戊糖磷酸途径的代谢减少，导致溶血。这些发现可为预防和治疗人类葡萄糖-6-磷酸脱氢酶缺乏症（“蚕豆病”）提供参考。
