Kojima Chie, Hirano Yusuke, Kono Kenji
Nanoscience and Nanotechnology Research Center, Research Institutes for the Twenty First Century, Osaka Prefecture University, Osaka, Japan.
Methods Enzymol. 2009;464:131-45. doi: 10.1016/S0076-6879(09)64007-6.
Liposomes have been widely used as drug carriers. Visible liposomes have recently become more attractive as drug carriers in personalized medicine. Gold nanoparticles (Au NPs) have unique size- and shape-dependent properties based on their surface plasmon resonance. They can be visualized by computed tomography (CT) and laser optoacoustic imaging. In addition, their photothermogenic properties are useful for photothermal therapy and photoresponsive drug release from liposomes. Therefore, complexation of liposomes with Au NPs is of considerable interest. There are three types of complex: Liposomes containing Au NPs in the inner phase, liposomes with Au NPs at the lipid membrane, and liposomes modified with Au NPs on the surface. This chapter focuses on the preparation and characterization of the third type of complex that is prepared by direct mixing of a Au NP dispersion with a liposome suspension.
脂质体已被广泛用作药物载体。可见脂质体最近在个性化医疗中作为药物载体变得更具吸引力。金纳米颗粒（Au NPs）基于其表面等离子体共振具有独特的尺寸和形状依赖性特性。它们可以通过计算机断层扫描（CT）和激光光声成像进行可视化。此外，它们的光热特性可用于光热疗法和脂质体的光响应药物释放。因此，脂质体与金纳米颗粒的复合备受关注。有三种类型的复合物：内相中含有金纳米颗粒的脂质体、脂质膜上带有金纳米颗粒的脂质体以及表面用金纳米颗粒修饰的脂质体。本章重点介绍通过将金纳米颗粒分散体与脂质体悬浮液直接混合制备的第三种复合物的制备和表征。
