Kojima Chie, Hirano Yusuke, Yuba Eiji, Harada Atsushi, Kono Kenji
Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, Japan.
Colloids Surf B Biointerfaces. 2008 Oct 15;66(2):246-52. doi: 10.1016/j.colsurfb.2008.06.022. Epub 2008 Jul 9.
Gold nanoparticles (Au NPs), which are extremely useful materials for imaging and photothermal therapy, typically require a drug delivery system to transport them to the affected tissue and into the cells. Since liposomes are approved as drug carriers, complexes of liposomes with Au NPs were considered ideal solutions to deliver Au NPs to the target site in vivo. In this study, we prepared complexes of various liposomes with Au NPs via physical absorption and characterized them. The time dependency of the surface plasmon resonance of this complex, which is a unique property of Au NPs, shows that the liposomes promote the formation of stable dispersions of Au NPs under isotonic conditions, even though intact Au NPs aggregate immediately. From a release assay of calcein from liposomes and transmission electron microscopy analysis, the Au NPs were complexed with liposomes without membrane disruption. These complexes could be formed by using cationic liposomes and polyethylene glycol-modified liposomes, as well as by using phosphatidylcholine liposomes, which are useful for drug and gene delivery. We proposed this kind of complex as a nanomedicine with diagnostic and therapeutic ability.
金纳米颗粒（Au NPs）是用于成像和光热治疗的极其有用的材料，通常需要药物递送系统将它们运输到受影响的组织并进入细胞。由于脂质体被批准为药物载体，脂质体与Au NPs的复合物被认为是在体内将Au NPs递送至靶位点的理想解决方案。在本研究中，我们通过物理吸附制备了各种脂质体与Au NPs的复合物并对其进行了表征。这种复合物的表面等离子体共振的时间依赖性是Au NPs的独特性质，表明脂质体在等渗条件下促进了Au NPs稳定分散体的形成，尽管完整的Au NPs会立即聚集。通过脂质体中钙黄绿素的释放测定和透射电子显微镜分析，Au NPs与脂质体复合而没有膜破坏。这些复合物可以通过使用阳离子脂质体和聚乙二醇修饰的脂质体以及通过使用对药物和基因递送有用的磷脂酰胆碱脂质体来形成。我们提出这种复合物作为具有诊断和治疗能力的纳米药物。
