Banchelli Martina, Bombelli Francesca Baldelli, Berti Debora, Baglioni Piero
Department of Chemistry and CSGI, University of Florence, Sesto Fiorentino, Florence, Italy.
Methods Enzymol. 2009;464:249-77. doi: 10.1016/S0076-6879(09)64013-1.
This chapter reports on the design, preparation, and characterization of liposomes decorated with synthetic lipid-oligonucleotide conjugates. Several key parameters should be considered for a successful preparation of these functional nanostructures that can be employed further as building blocks in DNA-directed assembly of nano-objects. These parameters are reviewed explicitly in this report and their contributions are discussed.