RNA 指导的 DNA 甲基化和植物发育需要一种保守的转录调节因子。
A conserved transcriptional regulator is required for RNA-directed DNA methylation and plant development.
作者信息
He Xin-Jian, Hsu Yi-Feng, Zhu Shihua, Liu Hai-Liang, Pontes Olga, Zhu Jianhua, Cui Xinping, Wang Co-Shine, Zhu Jian-Kang
机构信息
Institute for Integrative Genome Biology and Department of Botany and Plant Sciences, University of California at Riverside, Riverside, California 92521, USA.
出版信息
Genes Dev. 2009 Dec 1;23(23):2717-22. doi: 10.1101/gad.1851809. Epub 2009 Nov 10.
Abstract
RNA-directed DNA methylation (RdDM) is a conserved mechanism for epigenetic silencing of transposons and other repetitive elements. We report that the rdm4 (RNA-directed DNA Methylation4) mutation not only impairs RdDM, but also causes pleiotropic developmental defects in Arabidopsis. Both RNA polymerase II (Pol II)- and Pol V-dependent transcripts are affected in the rdm4 mutant. RDM4 encodes a novel protein that is conserved from yeast to humans and interacts with Pol II and Pol V in plants. Our results suggest that RDM4 functions in epigenetic regulation and plant development by serving as a transcriptional regulator for RNA Pol V and Pol II, respectively.
摘要
RNA 指导的 DNA 甲基化（RdDM）是转座子和其他重复元件表观遗传沉默的保守机制。我们报道，rdm4（RNA 指导的 DNA 甲基化 4）突变不仅损害 RdDM，还在拟南芥中导致多效性发育缺陷。rdm4 突变体中依赖 RNA 聚合酶 II（Pol II）和 Pol V 的转录本均受到影响。RDM4 编码一种从酵母到人类都保守的新型蛋白质，并在植物中与 Pol II 和 Pol V 相互作用。我们的结果表明，RDM4 分别作为 RNA Pol V 和 Pol II 的转录调节因子，在表观遗传调控和植物发育中发挥作用。
相似文献
1
A conserved transcriptional regulator is required for RNA-directed DNA methylation and plant development.RNA 指导的 DNA 甲基化和植物发育需要一种保守的转录调节因子。
Genes Dev. 2009 Dec 1;23(23):2717-22. doi: 10.1101/gad.1851809. Epub 2009 Nov 10.
2
RNA-directed DNA methylation and plant development require an IWR1-type transcription factor.RNA 指导的 DNA 甲基化和植物发育需要 IWR1 型转录因子。
EMBO Rep. 2010 Jan;11(1):65-71. doi: 10.1038/embor.2009.246. Epub 2009 Dec 11.
3
NRPD4, a protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerases IV and V and is required for RNA-directed DNA methylation.NRPD4是一种与RNA聚合酶II的RPB4亚基相关的蛋白质，是RNA聚合酶IV和V的一个组成部分，是RNA指导的DNA甲基化所必需的。
Genes Dev. 2009 Feb 1;23(3):318-30. doi: 10.1101/gad.1765209.
4
RNA polymerases IV and V influence the 3' boundaries of Polymerase II transcription units in Arabidopsis.RNA 聚合酶 IV 和 V 影响拟南芥中聚合酶 II 转录单位的 3' 边界。
RNA Biol. 2018 Feb 1;15(2):269-279. doi: 10.1080/15476286.2017.1409930. Epub 2017 Dec 21.
5
A Pol V-mediated silencing, independent of RNA-directed DNA methylation, applies to 5S rDNA.一种不依赖于RNA指导的DNA甲基化的Pol V介导的沉默作用适用于5S核糖体DNA。
PLoS Genet. 2009 Oct;5(10):e1000690. doi: 10.1371/journal.pgen.1000690. Epub 2009 Oct 16.
6
The initiation of epigenetic silencing of active transposable elements is triggered by RDR6 and 21-22 nucleotide small interfering RNAs.启动子相关的转录元件的表观遗传沉默是由 RDR6 和 21-22 核苷酸的小干扰 RNA 触发的。
Plant Physiol. 2013 May;162(1):116-31. doi: 10.1104/pp.113.216481. Epub 2013 Mar 29.
7
The RNA silencing enzyme RNA polymerase v is required for plant immunity.RNA 沉默酶 RNA 聚合酶 v 是植物免疫所必需的。
PLoS Genet. 2011 Dec;7(12):e1002434. doi: 10.1371/journal.pgen.1002434. Epub 2011 Dec 29.
8
A protein complex required for polymerase V transcripts and RNA- directed DNA methylation in Arabidopsis.在拟南芥中，聚合酶 V 转录物和 RNA 指导的 DNA 甲基化所需的蛋白质复合物。
Curr Biol. 2010 May 25;20(10):951-6. doi: 10.1016/j.cub.2010.03.062. Epub 2010 Apr 22.
9
A conserved Pol II elongator SPT6L mediates Pol V transcription to regulate RNA-directed DNA methylation in Arabidopsis.一个保守的 Pol II 延伸因子 SPT6L 介导 Pol V 转录以调节拟南芥中的 RNA 指导的 DNA 甲基化。
Nat Commun. 2024 May 25;15(1):4460. doi: 10.1038/s41467-024-48940-8.
10
SHH1, a homeodomain protein required for DNA methylation, as well as RDR2, RDM4, and chromatin remodeling factors, associate with RNA polymerase IV.SHH1，一种同源域蛋白，对于 DNA 甲基化，以及 RDR2、RDM4 和染色质重塑因子，与 RNA 聚合酶 IV 相关。
PLoS Genet. 2011 Jul;7(7):e1002195. doi: 10.1371/journal.pgen.1002195. Epub 2011 Jul 21.
引用本文的文献
1
REM transcription factors and GDE1 shape the DNA methylation landscape through the recruitment of RNA polymerase IV transcription complexes.REM转录因子和GDE1通过募集RNA聚合酶IV转录复合物塑造DNA甲基化图谱。
Nat Cell Biol. 2025 Jun 27. doi: 10.1038/s41556-025-01691-0.
2
A conserved Pol II elongator SPT6L mediates Pol V transcription to regulate RNA-directed DNA methylation in Arabidopsis.一个保守的 Pol II 延伸因子 SPT6L 介导 Pol V 转录以调节拟南芥中的 RNA 指导的 DNA 甲基化。
Nat Commun. 2024 May 25;15(1):4460. doi: 10.1038/s41467-024-48940-8.
3
RETINOBLASTOMA-RELATED interactions with key factors of the RNA-directed DNA methylation (RdDM) pathway and its influence on root development.视网膜母细胞瘤相关的 RNA 导向的 DNA 甲基化（RdDM）途径关键因子的相互作用及其对根发育的影响。
Planta. 2023 Apr 30;257(6):105. doi: 10.1007/s00425-023-04135-x.
4
Systematic genome-wide and expression analysis of RNA-directed DNA methylation pathway genes in grapes predicts their involvement in multiple biological processes.葡萄中RNA定向DNA甲基化途径基因的全基因组系统分析和表达分析预测了它们参与多种生物学过程。
Front Plant Sci. 2022 Dec 9;13:1089392. doi: 10.3389/fpls.2022.1089392. eCollection 2022.
5
A naturally-occurring phenomenon of flower color change during flower development in .在……中花朵发育过程中自然发生的花色变化现象。
你提供的原文似乎不完整，句末的“in.”后面应该还有具体内容。
Front Plant Sci. 2022 Nov 15;13:1072185. doi: 10.3389/fpls.2022.1072185. eCollection 2022.
6
Developing Genetic Engineering Techniques for Control of Seed Size and Yield.开发控制种子大小和产量的遗传工程技术。
Int J Mol Sci. 2022 Oct 31;23(21):13256. doi: 10.3390/ijms232113256.
7
Molecular and Physiological Mechanisms to Mitigate Abiotic Stress Conditions in Plants.植物缓解非生物胁迫条件的分子与生理机制
Life (Basel). 2022 Oct 19;12(10):1634. doi: 10.3390/life12101634.
8
Comparative DNA Methylome of Phytoplasma Associated Retrograde Metamorphosis in Sesame ( L.).芝麻（L.）中与植原体相关的逆行变态的比较DNA甲基化组
Biology (Basel). 2022 Jun 23;11(7):954. doi: 10.3390/biology11070954.
9
Multifaceted roles of RNA polymerase IV in plant growth and development.RNA聚合酶IV在植物生长发育中的多方面作用。
J Exp Bot. 2020 Oct 7;71(19):5725-5732. doi: 10.1093/jxb/eraa346.
10
A Role for PICKLE in the Regulation of Cold and Salt Stress Tolerance in Arabidopsis.PICKLE在拟南芥抗寒和耐盐胁迫调控中的作用
Front Plant Sci. 2019 Jul 9;10:900. doi: 10.3389/fpls.2019.00900. eCollection 2019.
本文引用的文献
1
An effector of RNA-directed DNA methylation in arabidopsis is an ARGONAUTE 4- and RNA-binding protein.拟南芥中RNA定向DNA甲基化的一个效应因子是一种AGO4和RNA结合蛋白。
Cell. 2009 May 1;137(3):498-508. doi: 10.1016/j.cell.2009.04.028.
2
RNA-mediated chromatin-based silencing in plants.植物中基于染色质的RNA介导的沉默
Curr Opin Cell Biol. 2009 Jun;21(3):367-76. doi: 10.1016/j.ceb.2009.01.025. Epub 2009 Feb 23.
3
NRPD4, a protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerases IV and V and is required for RNA-directed DNA methylation.NRPD4是一种与RNA聚合酶II的RPB4亚基相关的蛋白质，是RNA聚合酶IV和V的一个组成部分，是RNA指导的DNA甲基化所必需的。
Genes Dev. 2009 Feb 1;23(3):318-30. doi: 10.1101/gad.1765209.
4
Subunit compositions of the RNA-silencing enzymes Pol IV and Pol V reveal their origins as specialized forms of RNA polymerase II.RNA沉默酶Pol IV和Pol V的亚基组成揭示了它们作为RNA聚合酶II特殊形式的起源。
Mol Cell. 2009 Jan 30;33(2):192-203. doi: 10.1016/j.molcel.2008.12.015. Epub 2008 Dec 24.
5
An atypical RNA polymerase involved in RNA silencing shares small subunits with RNA polymerase II.一种参与RNA沉默的非典型RNA聚合酶与RNA聚合酶II共享小亚基。
Nat Struct Mol Biol. 2009 Jan;16(1):91-3. doi: 10.1038/nsmb.1539. Epub 2008 Dec 14.
6
Noncoding transcription by RNA polymerase Pol IVb/Pol V mediates transcriptional silencing of overlapping and adjacent genes.RNA聚合酶Pol IVb/Pol V介导的非编码转录调控重叠基因和相邻基因的转录沉默。
Cell. 2008 Nov 14;135(4):635-48. doi: 10.1016/j.cell.2008.09.035.
7
The transition from transcriptional initiation to elongation.从转录起始到延伸的转变。
Curr Opin Genet Dev. 2008 Apr;18(2):130-6. doi: 10.1016/j.gde.2007.12.008. Epub 2008 Feb 20.
8
Transgenerational stability of the Arabidopsis epigenome is coordinated by CG methylation.拟南芥表观基因组的跨代稳定性由CG甲基化协调。
Cell. 2007 Sep 7;130(5):851-62. doi: 10.1016/j.cell.2007.07.007.
9
The molecular basis of eukaryotic transcription.真核生物转录的分子基础。
Proc Natl Acad Sci U S A. 2007 Aug 7;104(32):12955-61. doi: 10.1073/pnas.0704138104. Epub 2007 Aug 1.
10
Role of Arabidopsis AGO6 in siRNA accumulation, DNA methylation and transcriptional gene silencing.拟南芥AGO6在小干扰RNA积累、DNA甲基化及转录基因沉默中的作用。
EMBO J. 2007 Mar 21;26(6):1691-701. doi: 10.1038/sj.emboj.7601603. Epub 2007 Mar 1.
Zotero 插件