National Livestock Breeding Center, Nishigo, Fukushima 961-8511, Japan.
Poult Sci. 2009 Dec;88(12):2512-7. doi: 10.3382/ps.2009-00331.
We performed candidate gene analysis to identify SNP in the chicken ovocalyxin-32 (OCX-32) gene in the F(2) resource population, to develop a PCR-RFLP method for genotyping and to evaluate the associations of the gene polymorphism with egg production traits. The F(2) resource population-comprising 272 chickens-was obtained by crossing White Leghorn (WL) males and Rhode Island Red (RIR) females. They were measured for egg production traits and used for candidate gene analysis. Among parental individuals of the F(2) population, 2 novel nonsynonymous polymorphisms (c.267T>G and c.494A>C) and 1 known nonsynonymous polymorphism (c.381G>C) in the coding sequences of the chicken OCX-32 gene were detected. The PCR-RFLP method was used for screening the chickens of the F(2) population. In parental populations, genotype c.267T>G and c.494A>C were segregated within WL and RIR breeds, respectively, but genotype c.381G>C was breed-specific SNP between WL and RIR breeds. A total of 4 haplotypes were constructed based on the 3 SNP in parental populations, and there was no recombination between c.267T>G and c.494A>C. There was a significant association (P < 0.05) between the OCX-32 gene SNP and egg production traits, but there was no significant association between the haplotypes of the OCX-32 gene and egg production traits in the F(2) population. In the present study, there was the most significant association between c.381G>C of the OCX-32 gene and rate of egg production. The current study is the first step to confirm the relationship between OCX-32 gene polymorphisms and egg production traits.
我们对 F2 资源群体中的鸡卵清蛋白-32（OCX-32）基因进行了候选基因分析，以鉴定 SNP，开发用于基因分型的 PCR-RFLP 方法，并评估基因多态性与产蛋性状的相关性。F2 资源群体由 272 只鸡组成，是通过杂交白来航鸡（WL）雄性和罗得岛红（RIR）雌性获得的。它们被测量产蛋性状，并用于候选基因分析。在 F2 群体的亲本个体中，在鸡 OCX-32 基因的编码序列中检测到 2 个新的非同义多态性（c.267T>G 和 c.494A>C）和 1 个已知的非同义多态性（c.381G>C）。使用 PCR-RFLP 方法筛选 F2 群体的鸡。在亲本群体中，基因型 c.267T>G 和 c.494A>C 分别在 WL 和 RIR 品种内分离，但基因型 c.381G>C 是 WL 和 RIR 品种之间的特有 SNP。基于 3 个 SNP 在亲本群体中构建了 4 个单倍型，并且 c.267T>G 和 c.494A>C 之间没有重组。OCX-32 基因 SNP 与产蛋性状之间存在显著关联（P<0.05），但在 F2 群体中，OCX-32 基因的单倍型与产蛋性状之间没有显著关联。在本研究中，OCX-32 基因的 c.381G>C 与产蛋率之间存在最显著的关联。本研究是首次确认 OCX-32 基因多态性与产蛋性状之间的关系。
