肠外营养减轻大鼠雨蛙肽诱导的胰腺炎。
Total parenteral nutrition attenuates cerulein-induced pancreatitis in rats.
机构信息
Department of Surgery, University of Wisconsin, Madison, WI 53706, USA.
出版信息
Pancreas. 2010 Apr;39(3):377-84. doi: 10.1097/MPA.0b013e3181bb908e.
OBJECTIVES
Our aim was to determine if total parenteral nutrition (TPN)-induced pancreatic atrophy and Hsp70 expression attenuates cerulein-induced pancreatitis in rats.
METHODS
Rats were randomized to a 7-day course of saline infusion plus a semipurified diet or TPN, with or without an intravenous cerulein injection or vehicle on day 7, and killed 1 or 6 hours after the injection. Based on a pilot study, 1 hour was the primary time point. Pancreatic atrophy was determined by mass, protein, and DNA contents. Pancreatic heat shock protein 70 (Hsp70) expression was measured by Western analysis. Histological examination of the pancreas assessed for edema, inflammation, vacuolization, and apoptosis. Serum amylase activity was measured using the Phadebas assay. Pancreatic trypsinogen activation was measured using a fluorometric substrate assay.
RESULTS
The saline-infused rats fed orally gained significantly more weight than TPN rats. The TPN decreased the pancreatic mass and protein content and the protein-DNA ratio and increased the pancreatic DNA content compared with the saline. The TPN increased the pancreatic Hsp70 expression by 91% compared with the saline. The TPN reduced the cerulein-induced pancreatic histological edema, the vacuolization, and the inflammation compared with the saline. The increase in the serum amylase level after cerulein injection was significantly attenuated, and trypsinogen activation was reduced in TPN animals compared with the saline group.
CONCLUSIONS
Lack of luminal nutrients with a 7-day course of TPN provides moderate protection against cerulein-induced pancreatitis in rats.
目的
本研究旨在探讨全肠外营养（TPN）诱导的胰腺萎缩和热休克蛋白 70（Hsp70）表达是否会减轻大鼠胰腺腺泡细胞中雨蛙肽诱导的胰腺炎。
方法
将大鼠随机分为 7 天生理盐水输注加半纯化饮食组或 TPN 组，第 7 天给予或不给予静脉注射雨蛙肽或载体，并在注射后 1 或 6 小时处死。根据一项初步研究，1 小时是主要的时间点。通过质量、蛋白质和 DNA 含量来确定胰腺萎缩程度。通过 Western 分析测定胰腺 Hsp70 表达。用组织学检查评估胰腺水肿、炎症、空泡化和细胞凋亡情况。使用 Phadebas 测定法测定血清淀粉酶活性。用荧光底物法测定胰腺胰蛋白酶原激活情况。
结果
口服生理盐水喂养的大鼠体重明显增加，而 TPN 喂养的大鼠体重减轻。与生理盐水组相比，TPN 降低了胰腺质量和蛋白质含量以及蛋白质-DNA 比值，并增加了胰腺 DNA 含量。与生理盐水组相比，TPN 使胰腺 Hsp70 表达增加了 91%。与生理盐水组相比，TPN 减轻了雨蛙肽诱导的胰腺组织水肿、空泡化和炎症。与生理盐水组相比，TPN 动物注射雨蛙肽后血清淀粉酶水平的升高明显减轻，胰蛋白酶原激活减少。
结论
7 天的 TPN 缺乏肠腔营养会对大鼠雨蛙肽诱导的胰腺炎提供一定程度的保护。
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