Department of Gastroenterology and Hepatology, Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing, 100853, China.
Mol Biol Rep. 2013 Feb;40(2):1443-9. doi: 10.1007/s11033-012-2187-6. Epub 2012 Oct 21.
Recent studies have indicated that pre-induction of heat shock protein 70 (HSP70) expression in the pancreas protects against secretagogue-induced pancreatitis. In those studies, the HSP70 was mostly induced by unfeasible conditions. The aim of this current study was to investigate the effect of peritoneal lavage with hot 0.9 % saline (42 °C) on the pancreatic expression of HSP70 and its protective effect on cerulein-induced acute pancreatitis in rats. Male Wistar rats were peritoneally lavaged with 0.9 % saline at 42 °C for 30 min. HSP70 expression was evaluated by western blotting analysis. Prior peritoneal lavages with hot and warm saline were performed. Acute pancreatitis was induced by administration of intraperitoneal injection of cerulein (20 μg/kg) four times, and its severity was assessed by measuring serum amylase, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and trypsinogen activation peptide (TAP) levels. Pancreatic sections were stained with hematoxylin and eosin for histological evaluation. Peritoneal lavage with hot 0.9 % saline increased intrapancreatic HSP70 expression and ameliorated the cerulein-induced pancreatitis in rats, judged by the significantly reduced serum amylase, TNF-α, and IL-6 concentrations; histopathological scores, and serum TAP levels. Peritoneal lavage with hot 0.9 % saline can induce HSP70 expression and prevent cerulein-induced acute pancreatitis in rats. The results suggest that HSP70 protects against cerulein-induced pancreatitis by preventing proinflammatory cytokine synthesis and trypsinogen activation during acute pancreatitis.
最近的研究表明，在胰腺中预先诱导热休克蛋白 70（HSP70）的表达可以防止促分泌素诱导的胰腺炎。在这些研究中，HSP70 主要是通过不可行的条件诱导的。本研究旨在探讨用 42°C 的热 0.9%盐水进行腹腔灌洗对 HSP70 在胰腺中的表达及其对大鼠雨蛙肽诱导的急性胰腺炎的保护作用。雄性 Wistar 大鼠用 42°C 的 0.9%盐水进行 30 分钟的腹腔灌洗。通过 Western blot 分析评估 HSP70 的表达。进行了热和温盐水的预先腹腔灌洗。通过腹腔内注射雨蛙肽（20μg/kg）四次诱导急性胰腺炎，并通过测量血清淀粉酶、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和胰蛋白酶原激活肽（TAP）水平来评估其严重程度。用苏木精和伊红对胰腺切片进行染色以进行组织学评估。用 0.9%盐水进行腹腔热灌洗可增加胰腺内 HSP70 的表达，并减轻大鼠的雨蛙肽诱导的胰腺炎，通过显著降低血清淀粉酶、TNF-α 和 IL-6 浓度；组织病理学评分和血清 TAP 水平来判断。用 0.9%盐水进行腹腔热灌洗可以诱导 HSP70 的表达并预防大鼠的雨蛙肽诱导的急性胰腺炎。结果表明，HSP70 通过在急性胰腺炎期间防止促炎细胞因子的合成和胰蛋白酶原的激活来保护雨蛙肽诱导的胰腺炎。
