Department of Chemistry, University of Hull, Cottingham Road, Hull, UK HU6 7RX.
Lab Chip. 2009 Dec 7;9(23):3430-2. doi: 10.1039/b913309g. Epub 2009 Sep 21.
A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was successfully extracted from buccal swabs using electro-osmotic pumping (EOP) coupled with in situ reagents contained within a 1.5% agarose gel matrix. The extracted DNA was of sufficient quantity and purity for polymerase chain reaction (PCR) amplification.
提出了一种新颖的 DNA 加载方法，用于在微流控系统上进行 DNA 提取。在离液盐溶液中的 DNA 被手动加载到与随后的洗涤和洗脱溶液正交的硅胶整体柱上。通过电渗流（EOP）与 1.5%琼脂糖凝胶基质中包含的原位试剂结合，成功地从口腔拭子中提取了 DNA。提取的 DNA 具有足够的数量和纯度，可用于聚合酶链反应（PCR）扩增。
