Shaw Kirsty J, Thain Lauren, Docker Peter T, Dyer Charlotte E, Greenman John, Greenway Gillian M, Haswell Stephen J
Department of Chemistry, University of Hull, Cottingham Road, Hull, HU6 7RX, United Kingdom.
Anal Chim Acta. 2009 Oct 12;652(1-2):231-3. doi: 10.1016/j.aca.2009.03.038. Epub 2009 Mar 31.
DNA extraction was carried out on silica-based monoliths within a microfluidic device. Solid-phase DNA extraction methodology was applied in which the DNA binds to silica in the presence of a chaotropic salt, such as guanidine hydrochloride, and is eluted in a low ionic strength solution, such as water. The addition of poly-A carrier RNA to the chaotropic salt solution resulted in a marked increase in the effective amount of DNA that could be recovered (25ng) compared to the absence of RNA (5ng) using the silica-based monolith. These findings confirm that techniques utilising nucleic acid carrier molecules can enhance DNA extraction methodologies in microfluidic applications.
在微流控装置内的基于二氧化硅的整体柱上进行DNA提取。采用了固相DNA提取方法，其中DNA在诸如盐酸胍等离液盐存在的情况下与二氧化硅结合，并在诸如水等低离子强度溶液中洗脱。与不使用RNA（5ng）相比，在离液盐溶液中添加聚A载体RNA后，使用基于二氧化硅的整体柱可回收的有效DNA量（25ng）显著增加。这些发现证实，利用核酸载体分子的技术可以增强微流控应用中的DNA提取方法。
