Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA.
Dalton Trans. 2009 Dec 7(45):10106-13. doi: 10.1039/b909137h. Epub 2009 Sep 14.
Photosystem I/dithiol molecular wire/nanoparticle bioconjugates have been shown to photocatalytically generate dihydrogen at linear rates when continuously illuminated. In an effort to maximize H2 production, the pH and ionic concentration of the solution, the mobility of the electron donor, the length and degree of saturation of the molecular wire, and the intensity of the light were systematically investigated. Optimal conditions included a solution buffered at pH 6.0, cross-linked plastocyanin, rebuilt spinach PS I, and the use of 1,4-benzenedithiol to connect PS I to the Pt nanoparticle. Illumination of this optimized Photosystem I/dithiol molecular wire/nanoparticle bioconjugate at a light intensity of 70 microE m(-2) s(-1) generated a measured one time H2 production rate of 312 micromol H2 mg Chl(-1) h(-1).
已证实，当连续受光照射时，光系统 I/二硫醇分子线/纳米粒子生物缀合物能以线性速率光催化生成氢气。为了最大限度地提高氢气产量，系统研究了溶液的 pH 值和离子浓度、电子供体的迁移率、分子线的长度和饱和度以及光的强度。最佳条件包括缓冲 pH 值为 6.0 的溶液、交联的质体蓝素、重建的菠菜 PS I 以及使用 1,4-苯二硫醇将 PS I 连接到 Pt 纳米颗粒上。以 70 μE m(-2) s(-1)的光强度照射这种优化的光系统 I/二硫醇分子线/纳米粒子生物缀合物，产生的实测氢气生成速率为 312 μmol H2 mg Chl(-1) h(-1)。
