The College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, People's Republic of China.
Parasitol Res. 2010 Jan;106(2):341-7. doi: 10.1007/s00436-009-1664-x. Epub 2009 Nov 11.
To identify Cryptosporidium species/genotypes in sheep in China and to elucidate the endemic transmission of cryptosporidiosis, a total of 1,701 fecal samples from five farms in four prefectures in Henan Province (central China) were examined. Eighty-two Cryptosporidium-positive samples were analyzed by polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis of the small subunit (SSU) rRNA gene and PCR analysis of the 60 kDa glycoprotein (gp60) gene, and 41 were further analyzed by DNA sequencing of the PCR products. The SSU rRNA-based PCR identified two Cryptosporidium species and one genotype, including the Cryptosporidium cervine genotype (74/82), Cryptosporidium andersoni (4/82), and Cryptosporidium xiaoi (4/82). The cervine genotype was found in all age groups, C. xiaoi in lambs, and C. andersoni in ewes. There were intragenetic differences in the SSU rRNA gene sequences of the Cryptosporidium cervine genotype and C. xiaoi. No Cryptosporidium parvum was detected by both SSU rRNA- and gp60-based PCR assays. These findings suggest that sheep are a potential source for zoonotic infections of the Cryptosporidium cervine genotype.
为了鉴定中国绵羊中的隐孢子虫种/基因型，并阐明隐孢子虫病的地方性传播，我们从河南省（中国中部）四个地区的五个农场共采集了 1701 份粪便样本。通过聚合酶链反应（PCR）-限制性片段长度多态性分析小亚基（SSU）rRNA 基因和 60 kDa 糖蛋白（gp60）基因的 PCR 分析，对 82 个隐孢子虫阳性样本进行了分析，其中 41 个进一步通过 PCR 产物的 DNA 测序进行了分析。基于 SSU rRNA 的 PCR 鉴定出了两种隐孢子虫和一种基因型，包括鹿隐孢子虫基因型（74/82）、牛隐孢子虫（4/82）和微小隐孢子虫（4/82）。鹿隐孢子虫基因型在所有年龄段均有发现，微小隐孢子虫见于羔羊，牛隐孢子虫见于母羊。鹿隐孢子虫基因型和微小隐孢子虫的 SSU rRNA 基因序列存在遗传差异。基于 SSU rRNA 和 gp60 的 PCR 检测均未检测到微小隐孢子虫。这些发现表明绵羊是鹿隐孢子虫基因型引起的人畜共患感染的潜在来源。
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