Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0315, USA.
J Assist Reprod Genet. 2009 Nov-Dec;26(11-12):613-9. doi: 10.1007/s10815-009-9362-2. Epub 2009 Nov 11.
The objectives were to test how the source of oocytes and semen impacted vitrification of large numbers of bovine oocytes and subsequent IVF and early embryo development to test procedures that may assist with assisted reproductive technologies in humans.
Bovine oocytes were vitrified from follicles of different diameters, small (< or =4 mm) and medium (4 to 10 mm), using nylon mesh. Oocytes were exposed to the cryoprotectant composed of 40% (v/v) ethylene glycol, 18% (w/v) Ficoll-70, and 0.3 M sucrose in three stepwise dilutions. Thawing was conducted with a series of 0.5, 0.25 and 0.125 M sucrose dilutions in 20% fetal bovine serum.
The cleavage (39.1% vs. 58.5%) and blastocyst rates (5.1% vs. 22.9%) were significantly lower for the vitrified oocytes. Follicle size had a significant impact on the development of embryos. Sires had significant effects on embryonic developmental rates.
We conclude that differences in development exist due to follicle source and sire used for IVF after vitrification.
本研究旨在探讨卵母细胞和精子的来源对大量牛卵母细胞玻璃化、随后的体外受精（IVF）和早期胚胎发育的影响，以检验可能有助于人类辅助生殖技术的方法。
采用尼龙网对不同直径（<或=4mm 和 4-10mm）的卵泡中的牛卵母细胞进行玻璃化处理，将卵母细胞暴露于由 40%（v/v）乙二醇、18%（w/v）Ficoll-70 和 0.3M 蔗糖组成的冷冻保护剂中，进行三步逐步稀释。解冻过程采用一系列 0.5、0.25 和 0.125M 蔗糖在 20%胎牛血清中的稀释液进行。
玻璃化的卵母细胞的卵裂率（39.1%比 58.5%）和囊胚率（5.1%比 22.9%）显著降低。卵泡大小对胚胎发育有显著影响。供体牛对胚胎发育率有显著影响。
我们得出结论，由于玻璃化后 IVF 中使用的卵泡来源和供体牛不同，因此存在发育差异。
