Hong Lei, Chen Xiang, Zhu Min, Ao Zheng, Tang Wen, Zhou Zhinan
Key Laboratory of Plateau Mountain Animal Genetics, Breeding and Reproduction, Ministry of Education, Guizhou University, Guiyang 550025, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou province, Guizhou University, Guiyang 550025, China.
Arch Anim Breed. 2022 Mar 10;65(1):105-111. doi: 10.5194/aab-65-105-2022. eCollection 2022.
Tissue inhibitor of metalloproteinase 1 (TIMP1) is associated with animal reproductive processes, such as follicular growth, ovulation, luteinization, and embryo development in mammals. The purposes of this study were to explore the expression and localization of TIMP1 in the ovarian tissues and determine the effect of TIMP1 on the function of granulosa cells and the association of TIMP1 with lambing-related genes of the goats. Immunohistochemical analysis showed that TIMP1 protein was strongly expressed by granulosa cells. Enzyme-linked immunosorbent assay (ELISA) results showed that TIMP1 overexpression promoted the secretion of estradiol of granulosa cells after 12, 24, and 48 h of transfection. Moreover, in vitro experiments indicated that TIMP1 had the ability to promote the cell proliferation and elevate the transcriptional levels of four genes associated with goat prolificacy, including , , , and , in granulosa cells. In conclusion, TIMP1 could be an important molecule in regulating reproductive performance of the goats by affecting estrogen secretion and cell proliferation, as well as the expression of lambing-related genes of granulosa cells in the goats.
金属蛋白酶组织抑制因子1（TIMP1）与动物生殖过程相关，如哺乳动物的卵泡生长、排卵、黄体化和胚胎发育。本研究的目的是探讨TIMP1在山羊卵巢组织中的表达和定位，并确定TIMP1对颗粒细胞功能的影响以及TIMP1与山羊产羔相关基因的关联。免疫组织化学分析表明，颗粒细胞强烈表达TIMP1蛋白。酶联免疫吸附测定（ELISA）结果显示，转染12、24和48小时后，TIMP1过表达促进了颗粒细胞雌二醇的分泌。此外，体外实验表明，TIMP1具有促进颗粒细胞增殖并提高与山羊繁殖力相关的四个基因（包括 、 、 和 ）转录水平的能力。总之，TIMP1可能是通过影响雌激素分泌、细胞增殖以及山羊颗粒细胞产羔相关基因的表达来调节山羊繁殖性能的重要分子。
