The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
Macromol Biosci. 2010 Feb 11;10(2):202-10. doi: 10.1002/mabi.200900230.
A temperature-responsive polymer poly{2-(dimethylamino)ethyl methacrylate-co-[cis-butenedioic anhydride-poly[(N-isopropylacrylamide)-co-(butyl methacrylate)]]} (PDMNIB) was synthesized by free radical polymerization. The polymer had a significant temperature-responsive behavior with a lower critical solution temperature (LCST) at 20 degrees C. Gel retardation assay showed that PDMNIB could efficiently interact with DNA. Dynamic light scattering (DLS) and zeta potential measurement indicated that the average sizes and the surface electric charges of the PDMNIB/DNA complexes could be changed by temperature. Due to the thermosensitive interaction between PDMNIB and DNA, the gene transfection efficiency of PDMNIB could be improved by temperature.
一种温敏聚合物聚{2-（二甲氨基）乙基甲基丙烯酸酯-co-[顺丁烯二酸酐-聚[（N-异丙基丙烯酰胺）-co-（甲基丙烯酸丁酯）]]}（PDMNIB）通过自由基聚合合成。该聚合物具有显著的温敏行为，其低临界溶液温度（LCST）为 20 摄氏度。凝胶阻滞实验表明，PDMNIB 可以与 DNA 有效相互作用。动态光散射（DLS）和zeta 电位测量表明，PDMNIB/DNA 复合物的平均粒径和表面电荷可以通过温度进行改变。由于 PDMNIB 与 DNA 之间的热相互作用，PDMNIB 的基因转染效率可以通过温度进行提高。
