Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University, PO Box 9502, 2333 CC Leiden, The Netherlands.
Phytochem Anal. 2010 Jan-Feb;21(1):4-13. doi: 10.1002/pca.1188.
Sample preparation in plant metabolomics is a fundamental and critical step with important consequences for the accuracy of results. Depending on the analytical tools and the metabolites of interest, sample preparation has to be decided. However, the various methods reported in the literature have many steps in common and consequently the practical considerations concerning the pros and cons are similar. In this review, each step of the sample preparation - harvesting, drying, extraction and purification - will be discussed in detail.
植物代谢组学中的样品制备是一个基本且关键的步骤，对结果的准确性有重要影响。根据分析工具和感兴趣的代谢物，需要决定样品制备方法。然而，文献中报道的各种方法有许多共同步骤，因此关于优缺点的实际考虑因素是相似的。在这篇综述中，将详细讨论样品制备的每一个步骤 - 收获、干燥、提取和纯化。
