Orthodontic Science, Department of Orofacial Development and Function, Tokyo Medical and Dental University, Tokyo, Japan.
Angle Orthod. 2010 Mar;80(2):309-15. doi: 10.2319/040109-185.1.
To analyze the age-dependent changes in nerve fibers immunoreactive to calcitonin gene-related peptide (CGRP-ir) in the periodontal ligaments of rats.
Thirty male Wistar-ST rats were divided into growing groups (5, 9, and 15 weeks of age) and aging groups (6, 12, and 24 months of age) (n = 5 in each group). Eight serial sagittal sections, 5 microm thick, were cut parallel to the distobuccal root of the maxillary right first molar. These tissues were stained with a rabbit monoclonal antibody against CGRP. The observation area was divided into three parts (mesial, apical, and distal) and observed using a light microscope.
CGRP-ir nerve fibers were primarily distributed in the apical periodontal ligament in the growing group, with significantly more fibers than in the aging group.
CGRP-ir nerve fibers in the periodontal ligament are dense during the growth period and decrease gradually with aging, indicating that CGRP may affect periodontal tissue with growth and aging.
分析大鼠牙周韧带中降钙素基因相关肽（CGRP-ir）免疫反应性神经纤维随年龄的变化。
30 只雄性 Wistar-ST 大鼠分为生长组（5、9 和 15 周龄）和老化组（6、12 和 24 月龄）（每组 5 只）。沿右上颌第一磨牙远颊根平行切 8 个连续矢状切片，厚 5 微米。这些组织用兔抗 CGRP 单克隆抗体染色。观察区分为近中、根尖和远中三部分，用光学显微镜观察。
CGRP-ir 神经纤维主要分布在生长组的根尖牙周韧带中，纤维数量明显多于老化组。
牙周韧带中的 CGRP-ir 神经纤维在生长期间密集，随年龄增长逐渐减少，提示 CGRP 可能影响生长和老化过程中的牙周组织。
