大鼠磨牙牙周韧带中降钙素基因相关肽免疫反应性神经纤维分布的变化与生长和老化的关系。
Changes in the distribution of nerve fibers immunoreactive to calcitonin gene-related peptide according to growth and aging in rat molar periodontal ligament.
机构信息
Orthodontic Science, Department of Orofacial Development and Function, Tokyo Medical and Dental University, Tokyo, Japan.
出版信息
Angle Orthod. 2010 Mar;80(2):309-15. doi: 10.2319/040109-185.1.
Abstract
OBJECTIVE
To analyze the age-dependent changes in nerve fibers immunoreactive to calcitonin gene-related peptide (CGRP-ir) in the periodontal ligaments of rats.
MATERIALS AND METHODS
Thirty male Wistar-ST rats were divided into growing groups (5, 9, and 15 weeks of age) and aging groups (6, 12, and 24 months of age) (n = 5 in each group). Eight serial sagittal sections, 5 microm thick, were cut parallel to the distobuccal root of the maxillary right first molar. These tissues were stained with a rabbit monoclonal antibody against CGRP. The observation area was divided into three parts (mesial, apical, and distal) and observed using a light microscope.
RESULTS
CGRP-ir nerve fibers were primarily distributed in the apical periodontal ligament in the growing group, with significantly more fibers than in the aging group.
CONCLUSIONS
CGRP-ir nerve fibers in the periodontal ligament are dense during the growth period and decrease gradually with aging, indicating that CGRP may affect periodontal tissue with growth and aging.
摘要
目的
分析大鼠牙周韧带中降钙素基因相关肽（CGRP-ir）免疫反应性神经纤维随年龄的变化。
材料与方法
30 只雄性 Wistar-ST 大鼠分为生长组（5、9 和 15 周龄）和老化组（6、12 和 24 月龄）（每组 5 只）。沿右上颌第一磨牙远颊根平行切 8 个连续矢状切片，厚 5 微米。这些组织用兔抗 CGRP 单克隆抗体染色。观察区分为近中、根尖和远中三部分，用光学显微镜观察。
结果
CGRP-ir 神经纤维主要分布在生长组的根尖牙周韧带中，纤维数量明显多于老化组。
结论
牙周韧带中的 CGRP-ir 神经纤维在生长期间密集，随年龄增长逐渐减少，提示 CGRP 可能影响生长和老化过程中的牙周组织。
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