Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
J Anim Physiol Anim Nutr (Berl). 2010 Jun;94(3):368-74. doi: 10.1111/j.1439-0396.2009.00917.x. Epub 2009 Nov 11.
The objective of this study was to investigate the effect of dietary phytate and phytase on the metabolic parameters of lipid, protein, enzyme, electrolyte in the blood or intestinal mucosa of broiler chickens. Diets containing phytate phosphorus (0.22% or 0.44%) with phytase supplementation (0, 500 or 1000 U/kg) were administrated to 504 Cobb chicks for 4 weeks. Results showed that the serum concentrations of total cholesterol (T-CHO), albumin, albumin/globulin, total superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC) and glutamic pyruvic transaminase (GPT) were decreased by 9-41% in high phytate diets (p < 0.05) and the concentrations of blood P, K, Fe, Cu, Zn and Mg were decreased by 4-14% for birds fed high phytate diets (p < 0.05), whereas inclusion of phytase compensated these adverse influences. In the duodenum, phytate decreased the level of T-AOC by 13% (p < 0.05), whereas phytase increased the levels of T-SOD, T-AOC and alkaline phosphatase (ALP) by 9-16% (p < 0.05). Also, in the jejunum, diets with high phytate showed lower activity of T-SOD, T-AOC and glutamic oxaloacetic transaminase (GOT) (p < 0.05), and phytase increased T-SOD, T-AOC and ALP (p < 0.05). However, phytase decreased transaminase activity in the low phytate basal diets (p < 0.05). This study suggests that dietary phytate can adversely interfere with the metabolisms of lipid and protein, as well as the antioxidation of blood and intestinal cells, while phytase supplementation may compensate these effects for broiler chickens.
本研究旨在探讨日粮植酸及植酸酶对肉鸡脂类、蛋白质代谢参数及血液和肠道黏膜中酶、电解质的影响。试验选用 504 只科宝肉鸡，采用 2×3 完全随机试验设计，分为 4 个处理，每个处理 6 个重复，每个重复 16 只鸡。对照组为基础日粮，植酸磷水平为 0.22%，不添加植酸酶；试验组在基础日粮中添加植酸酶 500 U/kg 和 1000 U/kg，植酸磷水平为 0.44%。试验期为 4 周。结果表明，高植酸日粮降低了血清总胆固醇（T-CHO）、白蛋白、白蛋白/球蛋白、总超氧化物歧化酶（T-SOD）、总抗氧化能力（T-AOC）和谷丙转氨酶（GPT）浓度（p<0.05），降低了血液磷、钾、铁、铜、锌和镁浓度（p<0.05），而添加植酸酶缓解了这些不利影响。在十二指肠中，植酸降低了 T-AOC 水平（p<0.05），而植酸酶提高了 T-SOD、T-AOC 和碱性磷酸酶（ALP）水平（p<0.05）。在空肠中，高植酸日粮降低了 T-SOD、T-AOC 和谷草转氨酶（GOT）活性（p<0.05），而植酸酶提高了 T-SOD、T-AOC 和 ALP 活性（p<0.05）。然而，植酸酶降低了低植酸基础日粮中转氨酶活性（p<0.05）。本研究表明，日粮植酸可对肉鸡脂类和蛋白质代谢及血液和肠道细胞抗氧化产生不利影响，而添加植酸酶可缓解这些影响。
