School of Livestock Science, Henan University of Science and Technology, Luoyang 471003, China.
Br J Nutr. 2010 Mar;103(6):862-8. doi: 10.1017/S0007114509992558. Epub 2009 Dec 10.
The objective of the present study was to investigate the effect of dietary phytate and microbial phytase on the lipase activity, lipid metabolism and mRNA expressions of fatty acid synthase (FASN) and leptin in broiler chickens. The study was conducted as a 2 x 3 factorial arrangement of treatments with phytate phosphorus at 0.20 and 0.40 % (added as the sodium phytate) and supplemental microbial phytase at 0, 500, or 1000 phytase units/kg. The results showed that phytase improved (P < 0.05) the growth performance and ileal digestibility of nutrients of broilers, but phytate had no effect (P>0.05) on these parameters, except the decrease (P < 0.01) in the digestibility of Ca. Phytate decreased (P < 0.05) the lipase activity, serum total cholesterol (T-CHO) and hepatic TAG, and elevated (P < 0.01) serum NEFA and HDL cholesterol. Phytase decreased (P < 0.05) serum NEFA, but increased (P < 0.01) serum T-CHO and hepatic TAG. Phytate and phytase also influenced (P < 0.01) the mRNA expressions of leptin in the liver. There were significant (P < 0.05) interactions of phytate and phytase on the concentrations of serum TAG and LDL cholesterol, hepatic NEFA and T-CHO, and the mRNA expressions of FASN. The results suggest that phytate and phytase can affect lipase activity and lipid metabolism of broiler chickens.
本研究旨在探讨日粮植酸和植酸酶对肉鸡脂肪酶活性、脂质代谢以及脂肪酸合成酶（FASN）和瘦素 mRNA 表达的影响。试验采用 2×3 因子设计，植酸磷水平设为 0.20%和 0.40%（以植酸钠形式添加），植酸酶添加量设为 0、500 和 1000 单位/千克。结果表明，植酸酶提高了肉鸡的生长性能和养分回肠消化率（P＜0.05），但植酸对这些参数没有影响（P＞0.05），除了钙消化率降低（P＜0.01）。植酸降低了脂肪酶活性、血清总胆固醇（T-CHO）和肝脏三酰甘油（TAG）含量，升高了血清非酯化脂肪酸（NEFA）和高密度脂蛋白胆固醇（HDL-C）含量（P＜0.05）。植酸酶降低了血清 NEFA 含量，但增加了血清 T-CHO 和肝脏 TAG 含量（P＜0.01）。植酸和植酸酶还影响了肝脏瘦素 mRNA 的表达（P＜0.01）。血清 TAG 和 LDL-C、肝脏 NEFA 和 T-CHO 以及 FASN mRNA 表达均存在植酸和植酸酶的显著互作（P＜0.05）。研究表明，植酸和植酸酶可以影响肉鸡的脂肪酶活性和脂质代谢。
