Department of Food Science and Nutrition, College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou, 310058, People's Republic of China.
Fish Physiol Biochem. 2012 Dec;38(6):1721-1728. doi: 10.1007/s10695-012-9669-2. Epub 2012 Jun 12.
The aim of this study was to explore the artificial hibernation of crucian carp for waterless preservation and to characterize the quality and biochemical properties during and after the hibernation. Anesthetized crucian carp using eugenol were stored at 8 °C with 90 % oxygen and 95-100 % relative humidity for 38 h and then transferred to fresh water to recover. Liquid loss and cooking loss had no significant changes (p > 0.05). The total volatile basic nitrogen content and 2-thiobarbituric acid value in hibernated fish were significantly higher (p < 0.05) than fresh and recovered groups. Serum cortisol, glutamic-oxaloacetic transaminase (GOT), alkaline phosphatase (AKP), and acid phosphatase (ACP) activities significantly increased (p < 0.05) during hibernation, while glutamic-pyruvic transaminase (GPT) had no significant change (p > 0.05). Both ACP and AKP activities decreased upon the fish recovered, but only the ACP activity returned to normal. However, there were increased serum glucose concentration, GOT and GPT activities in recovered fish. On the basis of these findings, it can be concluded that the artificially hibernated life of crucian carp was 38 h by the combination of anaesthetizing and low temperature. The muscle quality would not be influenced, and most of the stress responses would disappear after hibernated fish recovered.
本研究旨在探索鲫鱼的人工冬眠，以研究冬眠过程中和冬眠结束后鲫鱼的质量和生化特性。用丁香酚麻醉鲫鱼，在 8°C 下用 90%的氧气和 95-100%的相对湿度储存 38 小时，然后转移到淡水中恢复。液体损失和蒸煮损失没有显著变化（p>0.05）。冬眠鱼的总挥发性碱性氮含量和 2-硫代巴比妥酸值显著高于新鲜鱼和恢复鱼组（p<0.05）。血清皮质醇、谷草转氨酶（GOT）、碱性磷酸酶（AKP）和酸性磷酸酶（ACP）的活性在冬眠期间显著升高（p<0.05），而谷丙转氨酶（GPT）没有显著变化（p>0.05）。鱼恢复后，ACP 和 AKP 的活性均下降，但只有 ACP 的活性恢复正常。然而，恢复鱼的血清葡萄糖浓度、GOT 和 GPT 活性增加。基于这些发现，可以得出结论，通过麻醉和低温相结合，鲫鱼的人工冬眠寿命为 38 小时。肌肉质量不会受到影响，冬眠后鱼恢复后大部分应激反应会消失。
