基于快速流动的斑点免疫分析方法的建立及其在犬血清学诊断钩端螺旋体病中的应用。

Development of rapid flow-through-based dot-immunoassay for serodiagnosis of leptospirosis in dogs.

机构信息

Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai, India.

出版信息

Comp Immunol Microbiol Infect Dis. 2011 Jan;34(1):17-22. doi: 10.1016/j.cimid.2009.10.003. Epub 2009 Nov 10.

DOI:10.1016/j.cimid.2009.10.003

PMID:19906426

Abstract

An IgG-ELISA used recombinant antigen and a rapid flow-through enzyme immunoassay were developed for rapid screening of leptospiral antibodies in dogs using recombinant LipL41, which is one of the conserved outer membrane proteins in pathogenic leptospires as the coating antigen. Results from this study were compared with the standard microscopic agglutination test and found that the sensitivity and specificity of the enzyme-linked immunosorbent assay were 75.46% and 93.29% and whereas that of flow-through-based dot-immunobinding assay were 87.73% and 89.63%, respectively. Relative merits of these tests were also assessed. The flow-through-based dot-immunobinding assay was thus proved to be a valid screening test for canine leptospirosis.

摘要

建立了一种 IgG-ELISA，使用重组抗原和快速流动酶免疫测定法，使用重组 LipL41（一种致病性钩端螺旋体中保守的外膜蛋白）作为包被抗原，快速筛选犬钩端螺旋体抗体。将该研究的结果与标准显微镜凝集试验进行比较，发现酶联免疫吸附试验的灵敏度和特异性分别为 75.46%和 93.29%，而基于流动的斑点免疫结合测定法的灵敏度和特异性分别为 87.73%和 89.63%。还评估了这些测试的相对优点。因此，流动的斑点免疫结合测定法被证明是一种有效的犬钩端螺旋体病筛选试验。