Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai 600 007, Tamil Nadu, India.
Appl Biochem Biotechnol. 2013 Jan;169(2):431-7. doi: 10.1007/s12010-012-9973-4. Epub 2012 Dec 5.
Microscopic agglutination test (MAT) is the standard method for the diagnosis of leptospirosis, which is laborious and the interpretation of the results is subjective. The present work describes the use of recombinant-based IgG ELISA for the serodiagnosis of leptospirosis. We used recombinant outer membrane protein OmpL1 as an antigen for conducting IgG enzyme-linked immunosorbent assay (ELISA). A total of 475 canine serum samples were subjected to IgG ELISA; 294 sera were positive to ELISA, while 283 were positive to MAT. All samples that were positive to MAT were positive to ELISA also, however, few samples which were negative to MAT were positive to ELISA, which suggested that recombinant-based IgG ELISA showed 100 % sensitivity when compared to MAT. Thus, this present study showed that recombinant OmpL1-based IgG ELISA appears to be a better alternative to MAT for the diagnosis of leptospirosis and rOmpL1 protein could be used as a potential diagnostic antigen in different assay formats for leptospirosis.
显微镜凝集试验（MAT）是诊断钩端螺旋体病的标准方法，但该方法繁琐，结果的解释具有主观性。本研究描述了基于重组的 IgG ELISA 在钩端螺旋体病血清学诊断中的应用。我们使用重组外膜蛋白 OmpL1 作为抗原进行 IgG 酶联免疫吸附试验（ELISA）。共检测了 475 份犬血清样本，294 份血清 ELISA 阳性，283 份 MAT 阳性。所有 MAT 阳性的样本也都 ELISA 阳性，但少数 MAT 阴性的样本 ELISA 阳性，这表明重组 IgG ELISA 的敏感性与 MAT 相比达到 100%。因此，本研究表明，基于重组 OmpL1 的 IgG ELISA 似乎是 MAT 的更好替代方法，用于诊断钩端螺旋体病，rOmpL1 蛋白可作为不同钩端螺旋体病检测方法的潜在诊断抗原。
