结膜下苏拉明对兔角膜新生血管的作用。

The effect of subconjunctival suramin on corneal neovascularization in rabbits.

机构信息

Laboratory of Visual Science, St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

出版信息

Cornea. 2010 Jan;29(1):86-92. doi: 10.1097/ICO.0b013e3181ae91e3.

DOI:10.1097/ICO.0b013e3181ae91e3

PMID:19907290

Abstract

PURPOSE

To evaluate the effect of subconjunctival injection of suramin on corneal neovascularization in rabbits.

METHODS

Corneal neovascularization was induced by silk suturing of the corneal stroma in 40 eyes of 40 male New Zealand white rabbits. Five days after suture placement, all rabbits were randomly divided into 4 groups of 10 rabbits and were treated subconjunctivally with balanced salt solution 0.1 mL (group 1), suramin 0.1 mL (10 mg/mL and 100 mg/mL, groups 2 and 3, respectively), and bevacizumab 2.5 mg/0.1 mL (group 4). Digital photographs of eyes were obtained and analyzed on days 7, 14, and 28 after subconjunctival injections. In addition, vascular endothelial growth factor (VEGF) enzyme-linked immunosorbent assay (ELISA) and immunohistochemical analyses were used to estimate the level of VEGF and the expression of VEGF and basic FGF in neovascularized cornea, respectively.

RESULTS

The neovascularized area in control was increased significantly for 14 days after subconjunctival injection, but slightly decreased on day 28. On days 7 and 14, group 4 exhibited greater antiangiogenic effect than group 3, but group 3 exhibited greater antiangiogenic effect than group 4 on day 28. VEGF ELISA analysis showed the mean concentration of VEGF in group 4 was significantly lower than with other treatments for the first 14 days, but the mean concentration of VEGF in group 4 was similar to that with group 3 on day 28. Immunohistochemical analysis showed that the expressions of both VEGF and basic fibroblast growth factor (FGF) were reduced in group 3 and that bevacizumab reduced VEGF expression relative to basic FGF on day 28.

CONCLUSION

Subconjunctival suramin 100 mg/mL exhibited less antiangiogenic effect than bevacizumab 2.5 mg during the early period of treatment, but it had a longer effect than that of bevacizumab later. Therefore, the combination of subconjunctival bevacizumab and suramin may provide a more potent effect in early treatment as well as a longer antiangiogenic effect in neovascularized cornea.

摘要

目的

评价结膜下注射苏拉明对兔角膜新生血管的作用。

方法

通过丝线缝合角膜基质在 40 只雄性新西兰白兔的 40 只眼中诱导角膜新生血管。缝线放置后 5 天，所有兔子随机分为 4 组，每组 10 只兔子，分别用平衡盐溶液 0.1mL（第 1 组）、苏拉明 0.1mL（10mg/mL 和 100mg/mL，第 2 组和第 3 组）和贝伐单抗 2.5mg/0.1mL（第 4 组）结膜下注射。在结膜下注射后第 7、14 和 28 天，获取眼睛的数字照片并进行分析。此外，使用血管内皮生长因子（VEGF）酶联免疫吸附测定（ELISA）和免疫组织化学分析分别估计 VEGF 水平以及新生血管化角膜中 VEGF 和碱性成纤维细胞生长因子（bFGF）的表达。

结果

结膜下注射后第 14 天，对照组新生血管化区域明显增加，但第 28 天略有减少。第 7 天和第 14 天，第 4 组的抗血管生成作用强于第 3 组，但第 28 天第 3 组的抗血管生成作用强于第 4 组。VEGF ELISA 分析显示，第 4 组 VEGF 的平均浓度在前 14 天显著低于其他治疗组，但第 4 组 VEGF 的平均浓度在第 28 天与第 3 组相似。免疫组织化学分析显示，第 3 组 VEGF 和碱性成纤维细胞生长因子（bFGF）的表达均减少，贝伐单抗组与 bFGF 相比，VEGF 表达相对减少。