Wilson Robert H, Chandra Malavika, Scheiman James, Simeone Diane, McKenna Barbara, Purdy Julianne, Mycek Mary-Ann
Applied Physics Program, University of Michigan, Ann Arbor, MI 48109-2099, USA.
Opt Express. 2009 Sep 28;17(20):17502-16. doi: 10.1364/OE.17.017502.
An empirical model was developed to interpret differences in the experimentally measured reflectance and fluorescence spectra of freshly excised human pancreatic tissues: normal, adenocarcinoma, and pancreatitis (inflammation). The model provided the first quantitative links between spectroscopic measurements and histological characteristics in the human pancreas. The reflectance model enabled the first (to our knowledge) extraction of wavelength resolved absorption and reduced scattering coefficients for normal and diseased human pancreatic tissues. The fluorescence model employed reflectance information to extract attenuation free "intrinsic" endogenous fluorescence spectra from normal pancreatic tissue, pancreatic adenocarcinoma, and pancreatitis. The method developed is simple, intuitive, and potentially useful for a range of applications in optical tissue diagnostics. This approach is potentially applicable to in vivo studies, because it can account for the absorptive effects of blood in tissues.
建立了一个经验模型，以解释新鲜切除的人类胰腺组织（正常组织、腺癌组织和胰腺炎（炎症）组织）实验测量的反射光谱和荧光光谱之间的差异。该模型首次提供了人体胰腺光谱测量与组织学特征之间的定量联系。反射模型首次（据我们所知）提取了正常和患病人类胰腺组织的波长分辨吸收系数和约化散射系数。荧光模型利用反射信息从正常胰腺组织、胰腺腺癌和胰腺炎中提取无衰减的“固有”内源性荧光光谱。所开发的方法简单、直观，在光学组织诊断的一系列应用中可能很有用。这种方法可能适用于体内研究，因为它可以考虑组织中血液的吸收效应。
