基于乙醇脱氢酶基因内含子2的DNA序列对林鼠属的分子系统学研究
MOLECULAR SYSTEMATICS OF THE GENUS NEOTOMA BASED ON DNA SEQUENCES FROM INTRON 2 OF THE ALCOHOL DEHYDROGENASE GENE.
作者信息
Longhofer Lisa K, Bradley Robert D
机构信息
Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409-3131, USA (LKL, RDB), Museum of Texas Tech University, Lubbock, TX 79409-3191, USA (RDB).
出版信息
J Mammal. 2006;87(5):961-970. doi: 10.1644/05-MAMM-A-355R1.1.
Phylogenetic relationships were evaluated among 13 species of Neotoma based on DNA sequences from intron 2 of the nuclear alcohol dehydrogenase gene 1 (Adh1-I2). Sequences were analyzed using parsimony, likelihood, and Bayesian methods. Three major clades (I-III) consistently were recovered and relationships among taxa within 2 of the clades remained unchanged between analyses; however, relationships within clade III were largely unresolved. Average genetic divergence values were 2.12% among species, 4% between subgenera (Teonoma and Neotoma), and 5.1% between genera (Hodomys and Neotoma). Adh1-I2 sequences were concatenated with mitochondrial cytochrome-b sequences generated from the same individuals. Examination of the combined data resulted in a phylogeny whose topology was similar to that based only on cytochrome-b sequences.
基于核醇脱氢酶基因1（Adh1-I2）内含子2的DNA序列，对13种林鼠属物种的系统发育关系进行了评估。使用简约法、似然法和贝叶斯方法对序列进行了分析。始终得到了三个主要分支（I-III），并且在两次分析之间，其中两个分支内的分类单元之间的关系保持不变；然而，分支III内的关系在很大程度上尚未得到解决。物种之间的平均遗传差异值为2.12%，亚属（Teonoma和林鼠属）之间为4%，属（Hodomys和林鼠属）之间为5.1%。将Adh1-I2序列与从相同个体生成的线粒体细胞色素b序列连接起来。对合并数据的检查得出了一个系统发育树，其拓扑结构与仅基于细胞色素b序列的拓扑结构相似。
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