García-Escudero Ramón, Paramio Jesús M
Molecular Oncology Unit, Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT), Madrid, Spain.
Methods Mol Biol. 2010;585:171-81. doi: 10.1007/978-1-60761-380-0_13.
DNA microarray technology is a powerful tool for genome-wide gene expression analysis of biological samples including skin and epidermal keratinocytes. Here we review the methodology for expression profiling analysis of skin tissue or purified keratinocytes from mice. We explained the methodology and protocols for RNA preservation and purification, RNA quality and integrity tests, and DNA microarray technology types that can be used. Furthermore, using a data set of mice samples, we explained how to perform chip raw data preprocessing and normalization, differential expression analysis, gene-clustering and functional analysis of gene deregulation.
DNA微阵列技术是一种用于对包括皮肤和表皮角质形成细胞在内的生物样品进行全基因组基因表达分析的强大工具。在此，我们综述了对小鼠皮肤组织或纯化的角质形成细胞进行表达谱分析的方法。我们解释了RNA保存和纯化、RNA质量和完整性测试以及可使用的DNA微阵列技术类型的方法和方案。此外，我们利用小鼠样本数据集，解释了如何进行芯片原始数据预处理和标准化、差异表达分析、基因聚类以及基因失调的功能分析。
