Romano Rose-Anne, Sinha Satrajit
Department of Biochemistry, State University of New York at Buffalo, Buffalo, NY, USA.
Methods Mol Biol. 2010;585:287-302. doi: 10.1007/978-1-60761-380-0_20.
The ability to specifically manipulate gene expression in vivo using mouse models has been one of the most important advances in understanding gene function over the last few decades. Methods used to control gene activities in the mouse include gene targeting and transgenic approaches. While gene targeting methods have proven to be powerful genetic tools designed to eliminate gene function by creating a "knockout" or "null mutant," transgenic studies offer gain-of-function capabilities, expression of dominant negative or knockdown of specific target genes and thus have often served a complementary and useful role. This chapter provides an overview for the generation of transgenic mouse models to study important questions in skin biology by taking advantage of the tetracycline-inducible gene expression system.
在过去几十年里，利用小鼠模型在体内特异性操纵基因表达的能力一直是理解基因功能方面最重要的进展之一。用于控制小鼠基因活性的方法包括基因靶向和转基因方法。虽然基因靶向方法已被证明是强大的遗传工具，旨在通过创建“敲除”或“无效突变体”来消除基因功能，但转基因研究提供了功能获得能力、显性负性表达或特定靶基因的敲低，因此常常起到补充和有用的作用。本章概述了利用四环素诱导基因表达系统生成转基因小鼠模型，以研究皮肤生物学中的重要问题。
