Paquet-Fifield Sophie, Redvers Richard P, Pouliot Normand, Kaur Pritinder
Epithelial Stem Cell Biology Laboratory, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.
Methods Mol Biol. 2010;585:369-82. doi: 10.1007/978-1-60761-380-0_26.
This protocol describes a technically simple in vivo assay of long-term skin regeneration in human skin, providing a reliable method for epidermal tissue reconstitution using small numbers of several types of epithelial cells, from epithelial cell lines to primary epithelial stem cells and transplanted with or without prior culture. The model relies on the repopulation of devitalized rat tracheas by human keratinocyte suspensions following subcutaneous transplantation into immunodeficient mice. Here, we describe complete optimization and characterization of this model for robust regeneration of epithelium from cell suspensions of a limited number of primary human keratinocytes.
本方案描述了一种技术上简单的人体皮肤长期再生体内试验，提供了一种可靠的方法，可使用少量几种类型的上皮细胞（从上皮细胞系到原代表皮干细胞）进行表皮组织重建，且这些细胞可在培养或未培养的情况下进行移植。该模型依赖于将人角质形成细胞悬液皮下移植到免疫缺陷小鼠后，失活的大鼠气管被重新填充。在此，我们描述了该模型的完全优化和表征，以便从有限数量的原代人角质形成细胞悬液中实现上皮的强大再生。
