使用体内再生试验对小鼠表皮干细胞进行有限稀释分析。
Limiting dilution analysis of murine epidermal stem cells using an in vivo regeneration assay.
作者信息
Strachan Lauren R, Ghadially Ruby
机构信息
San Francisco VA Medical Center, University of California, San Francisco, CA, USA.
出版信息
Methods Mol Biol. 2010;585:421-32. doi: 10.1007/978-1-60761-380-0_29.
Abstract
Epidermal stem cells are of major importance for tissue homeostasis, wound repair, tumor initiation, and gene therapy. Here we describe an in vivo regeneration assay to test for the ability of keratinocyte progenitors to maintain an epidermis over the long-term in vivo. Limiting dilution analysis of epidermal repopulating units in this in vivo regeneration assay at sequential time points allows the frequency of short-term (transit amplifying cell) and long-term (stem cell) repopulating cells to be quantified.
摘要
表皮干细胞对于组织稳态、伤口修复、肿瘤起始和基因治疗至关重要。在此，我们描述了一种体内再生试验，以测试角质形成细胞祖细胞在体内长期维持表皮的能力。在该体内再生试验中，对连续时间点的表皮再殖单位进行极限稀释分析，可以量化短期（过渡扩增细胞）和长期（干细胞）再殖细胞的频率。
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Rapid adhesion to collagen isolates murine keratinocytes with limited long-term repopulating ability in vivo despite high clonogenicity in vitro.尽管在体外具有高克隆形成能力，但对胶原蛋白的快速黏附会分离出体内长期增殖能力有限的小鼠角质形成细胞。
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Plasticity of epidermal stem cells: survival in various environments.表皮干细胞的可塑性：在不同环境中的存活
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