Strachan Lauren R, Ghadially Ruby
San Francisco VA Medical Center, University of California, San Francisco, CA, USA.
Methods Mol Biol. 2010;585:421-32. doi: 10.1007/978-1-60761-380-0_29.
Epidermal stem cells are of major importance for tissue homeostasis, wound repair, tumor initiation, and gene therapy. Here we describe an in vivo regeneration assay to test for the ability of keratinocyte progenitors to maintain an epidermis over the long-term in vivo. Limiting dilution analysis of epidermal repopulating units in this in vivo regeneration assay at sequential time points allows the frequency of short-term (transit amplifying cell) and long-term (stem cell) repopulating cells to be quantified.
表皮干细胞对于组织稳态、伤口修复、肿瘤起始和基因治疗至关重要。在此，我们描述了一种体内再生试验，以测试角质形成细胞祖细胞在体内长期维持表皮的能力。在该体内再生试验中，对连续时间点的表皮再殖单位进行极限稀释分析，可以量化短期（过渡扩增细胞）和长期（干细胞）再殖细胞的频率。
