Molecular Oncology Unit, CIEMAT (ed 70A), Madrid, Spain.
Molecular Oncology, University Hospital 12 de Octubre, Research Institute 12 de Octubre i+12, Madrid, Spain.
Methods Mol Biol. 2020;2109:45-53. doi: 10.1007/7651_2019_234.
Epidermal stem cells are responsible for normal tissue homeostasis and contribute to tissue regeneration during injury. Several assays measuring stem cell frequency and function can be used to assess epidermal stem cell potential. However, the ultimate assay that accounts for stemness is the capacity to sustain in vivo long-term tissue regeneration and maintenance. We can use this type of analysis to interrogate whether a specific genetic alteration (e.g., activation or inactivation of any gene thought to be involved in stem cell quiescence or proliferation) confers increased or decreased stem cell potential.
表皮干细胞负责正常组织内稳态，并在损伤时有助于组织再生。有几种测量干细胞频率和功能的测定方法可用于评估表皮干细胞潜能。然而，最终测定干细胞特性的方法是维持体内长期组织再生和维持的能力。我们可以使用这种类型的分析来探究特定的遗传改变（例如，激活或失活任何被认为参与干细胞静止或增殖的基因）是否赋予了更高或更低的干细胞潜能。
