Institute of Physical and Theoretical Chemistry, Eberhard Karls University, 72076, Tübingen, Germany.
Anal Bioanal Chem. 2010 Jan;396(1):3-14. doi: 10.1007/s00216-009-3227-5. Epub 2009 Nov 12.
We present a general review of different microresonator structures and how they can be used in future device applications in modern analytical methods by tailoring the optical properties of single quantum emitters. The main emphasis is on the tunable lambda/2-Fabry-Perot-type microresonator which we used to obtain the results presented in this article. By varying the mirror distance the local mode structure of the electromagnetic field is altered and thus the radiative coupling of fluorescent single quantum emitters embedded inside the resonator to that field is changed, too. As a result a modification of the optical properties of these quantum emitters can be observed. We present experimental as well as theoretical results illustrating this effect. Furthermore, the developed resonator can be used to determine the longitudinal position of embedded emitters with an accuracy of lambda/60 by analyzing the excitation patterns of nano-sized fluorescent polymer spheres after excitation with a radially polarized doughnut mode laser beam. Finally, we will apply this resonator to a biological system and demonstrate the modification of Förster resonant energy transfer (FRET) efficiency by inhibiting the excited state energy transfer from the donor to the acceptor chromophore of a single DsRed protein.
我们介绍了不同微谐振器结构的综合评述,以及如何通过调整单个量子发射器的光学性质,将它们应用于现代分析方法中的未来器件应用中。重点介绍了可调谐的 lambda/2-Fabry-Perot 型微谐振器,我们使用该谐振器获得了本文中呈现的结果。通过改变反射镜的距离,可以改变电磁场的局部模式结构,从而改变嵌入谐振器内部的荧光单量子发射器与该场的辐射耦合。因此,可以观察到这些量子发射器光学性质的改变。我们展示了实验和理论结果来说明这一效果。此外,通过分析用径向偏振面包圈模式激光束激发后的纳米级荧光聚合物球体的激发模式,可以用这种开发的谐振器以 lambda/60 的精度确定嵌入发射器的纵向位置。最后,我们将该谐振器应用于生物系统,并通过抑制供体到单个 DsRed 蛋白的受体发色团的激发态能量转移,证明了Förster 共振能量转移(FRET)效率的改变。