Oral BioSciences, Faculty of Dentistry, University of Hong Kong.
Yeast. 2010 Jan;27(1):53-8. doi: 10.1002/yea.1732.
Candida kefyr is a common yeast species that can be found in fermented milk and cheeses. As a first step to developing a gene transfer system for C. kefyr, the orotidine-5'-phosphate decarboxylase (URA3) gene was cloned, using degenerate PCR and genome walking. The uninterrupted open reading frame of the C. kefyr URA3 gene spans 801 bp, corresponding to 267 amino acid residues. The functionality of the gene was confirmed by complementation of ura3 auxotrophs of C. albicans and Saccharomyces cerevisiae. Phylogenetic analysis of the deduced amino acid sequence indicated that it shares a high degree of homology with other Candida URA3 homologues.
卡氏酵母是一种常见的酵母，可在发酵乳和奶酪中发现。为了开发卡氏酵母的基因转移系统，我们使用简并 PCR 和基因组步移法克隆了乳清酸 5′-磷酸脱羧酶（URA3）基因。卡氏酵母 URA3 基因的连续开放阅读框跨越 801bp，对应 267 个氨基酸残基。通过对白色念珠菌和酿酒酵母 ura3 营养缺陷型的互补验证了该基因的功能。推导的氨基酸序列的系统发育分析表明，它与其他念珠菌 URA3 同源物具有高度的同源性。
