前列腺凋亡反应蛋白 4 在鼻咽癌中的广泛表达。

Widespread expression of prostate apoptosis response-4 in nasopharyngeal carcinoma.

机构信息

Institute of Life Science, College of Life Science, Tzu-Chi University, Hualien, Taiwan.

出版信息

Head Neck. 2010 Jul;32(7):877-85. doi: 10.1002/hed.21282.

DOI:10.1002/hed.21282

PMID:19908316

Abstract

BACKGROUND

Prostate apoptosis response-4 (Par-4) augments apoptosis in various tumors, either during apoptotic insult or by ectopic overexpression. However, investigation of Par-4 expression in nasopharyngeal carcinoma (NPC) is lacking.

METHODS

Specimens from patients with NPC, hypopharyngeal carcinoma (HPC), or oral cavity cancer were examined for Par-4 expression using immunohistochemistry. NPC cell proliferation and apoptosis were analyzed using immunohistochemical staining for Ki67, B-cell lymphoma 2 (Bcl-2), and in situ terminal deoxyribonucleotidyl transferase (TdT)-mediated biotin-16-dUTP nick end-labeling (TUNEL) assay, respectively.

RESULTS

Par-4 was ubiquitously expressed in NPC biopsies (96.2%, 25/26) and was significantly higher than in HPC (47.6%, 50/105, p < .0001) and oral cavity cancers (38.7%, 12/31, p < .0001). Remarkably, apoptosis of NPC cells was absent and Par-4 expression was associated with obvious expression of Bcl-2 and Ki67 in all patients tested with NPC.

CONCLUSIONS

Immunohistochemistry results showed widespread expression of Par-4 in NPC and revealed sustainable proliferation of NPC cells regardless of Par-4 expression.

摘要

背景

前列腺凋亡反应蛋白 4（Par-4）在各种肿瘤中增强细胞凋亡，无论是在凋亡损伤期间还是通过异位过表达。然而，关于鼻咽癌（NPC）中 Par-4 表达的研究还很缺乏。

方法

使用免疫组织化学方法检测来自 NPC、下咽癌（HPC）或口腔癌患者的标本中 Par-4 的表达。通过 Ki67、B 细胞淋巴瘤 2（Bcl-2）的免疫组织化学染色以及原位末端脱氧核苷酸转移酶（TdT）介导的生物素 16-dUTP 缺口末端标记（TUNEL）检测分别分析 NPC 细胞的增殖和凋亡。

结果

Par-4 在 NPC 活检标本中广泛表达（96.2%，25/26），明显高于 HPC（47.6%，50/105，p<.0001）和口腔癌（38.7%，12/31，p<.0001）。值得注意的是，在所有检测的 NPC 患者中，NPC 细胞的凋亡均不存在，并且 Par-4 表达与明显的 Bcl-2 和 Ki67 表达相关。