Ceroni Francesca, Furini Simone, Cavalcanti Silvio
Department of Electronics, Computer Sciences and Systems (DEIS), Cellular and Molecular Engineering Laboratory, University of Bologna, via Venezia 52, Cesena 47023, Italy.
Pac Symp Biocomput. 2010:409-20. doi: 10.1142/9789814295291_0043.
Synthetic biology aims to the rational design of gene circuits with predictable behaviours. Great efforts have been done so far to introduce in the field mathematical models that could facilitate the design of synthetic networks. Here we present a mathematical model of a synthetic gene-circuit with a negative feedback. The closed loop configuration allows the control of transcription by an inducer molecule (IPTG). Escherichia coli bacterial cells were transformed and expression of a fluorescent reporter (GFP) was measured for different inducer levels. Computer model simulations well reproduced the experimental induction data, using a single fitting parameter. Independent genetic components were used to assemble the synthetic circuit. The mathematical model here presented could be useful to predict how changes in these genetic components affect the behaviour of the synthetic circuit.
合成生物学旨在对具有可预测行为的基因回路进行合理设计。到目前为止，人们已经付出了巨大努力，在该领域引入能够促进合成网络设计的数学模型。在此，我们展示了一个具有负反馈的合成基因回路的数学模型。闭环配置允许通过诱导分子（异丙基-β-D-硫代半乳糖苷，IPTG）来控制转录。对大肠杆菌细胞进行转化，并针对不同的诱导剂水平测量荧光报告基因（绿色荧光蛋白，GFP）的表达。计算机模型模拟使用单个拟合参数很好地再现了实验诱导数据。使用独立的遗传元件来组装合成回路。这里展示的数学模型可能有助于预测这些遗传元件的变化如何影响合成回路的行为。
