Caballero David, Samitier Josep, Errachid Abdelhamid
Nanobioengineering group, Institute for Bioengineering of Catalonia, Barcelona Science Park, CIBER de Bioingeniería, Biomateriales y Nanomedicina and Department of Electronics, University of Barcelona, c/ Baldiri Reixac 10-12, 08028 Barcelona, Spain.
J Nanosci Nanotechnol. 2009 Nov;9(11):6478-82. doi: 10.1166/jnn.2009.1349.
Biological patterned surfaces having sub-micron scale resolution are of great importance in many fields of life science and biomedicine. Different techniques have been proposed for surface patterning at the nanoscale. However, most of them present some limitations regarding the patterned area size or are time-consuming. Micro/nanocontact printing is the most representative soft lithography-based technique for surface patterning at the nanoscale. Unfortunately, conventional micro/nanocontact printing also suffers from problems such as diffusion and stamp collapsing that limit pattern resolution. To overcome these problems, a simple way of patterning thiols under liquid media using submerged nanocontact printing (SnCP) over large areas (approximately cm2) achieving nanosize resolution is presented. The technique is also low cost and any special equipment neither laboratory conditions are required. Nanostructured poly(dimethyl siloxane) stamps are replicated from commercially available digital video disks. SnCP is used to stamp patterns of 200 nm 1-octadecanethiol lines in liquid media, avoiding ink diffusion and stamp collapsing, over large areas on gold substrates compared with conventional procedures. Atomic force microscopy measurements reveal that the patterns have been successfully transferred with high fidelity. This is an easy, direct, effective and low cost methodology for molecule patterning immobilization which is of interest in those areas that require nanoscale structures over large areas, such as tissue engineering or biosensor applications.
具有亚微米级分辨率的生物图案化表面在生命科学和生物医学的许多领域中都非常重要。已经提出了不同的技术用于纳米级表面图案化。然而，它们中的大多数在图案区域大小方面存在一些限制，或者耗时较长。微/纳米压印是用于纳米级表面图案化的最具代表性的基于软光刻的技术。不幸的是，传统的微/纳米压印也存在诸如扩散和印章塌陷等问题，这些问题限制了图案分辨率。为了克服这些问题，本文提出了一种在液体介质下使用浸没式纳米压印（SnCP）在大面积（约平方厘米）上图案化硫醇的简单方法，可实现纳米尺寸分辨率。该技术成本也很低，不需要任何特殊设备或实验室条件。纳米结构的聚二甲基硅氧烷印章是从市售数字视频光盘复制而来的。与传统方法相比，SnCP用于在液体介质中在金基板上大面积地压印200纳米的1-十八烷硫醇线图案，避免了油墨扩散和印章塌陷。原子力显微镜测量表明，图案已成功以高保真度转移。这是一种用于分子图案化固定的简单、直接、有效且低成本的方法，在那些需要大面积纳米级结构的领域，如组织工程或生物传感器应用中具有重要意义。
