Wan Y Z, Gao C, Luo H L, He F, Liang H, Li X L, Wang Y L
Key Laboratory of Advanced Ceramics and Machining Technology, Ministry of Education, Tianjin University, Tianjin 300072, PR China.
J Nanosci Nanotechnol. 2009 Nov;9(11):6494-500. doi: 10.1166/jnn.2009.1311.
It is believed that studies on the early hydroxyapatite (HAp) deposition on nano-sized substrates may possibly allow us to understand the formation mechanisms of biominerals at the molecular level. In this study, bacterial cellulose (BC) nanofibers were phosphorylated and used as nano-sized templates for early mineralization of calcium phosphate (Ca-P). To initiate mineralization the BC nanofibers were immersed in 1.5 times simulated body fluids (1.5 SBF) at 37 degreees C for varying periods of time. The deposited minerals on the nanofiber surfaces were characterized using scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transformed infrared spectroscopy (FTIR). SEM observations confirmed that early growth (at 4 h) of the Ca-P minerals was heterogeneous, which was followed by extensive spread of the minerals on the entire surfaces of the nanofibers. XRD and FTIR analyses indicated that octacalcium phosphate (OCP) was the precursor of HAp formed on BC nanofibers. Furthermore, HAp deposited on BC nanofibers elongated along the c axis. Nucleation and growth of the Ca-P minerals were analyzed in this paper.
据信，对纳米尺寸基底上早期羟基磷灰石（HAp）沉积的研究可能使我们能够在分子水平上理解生物矿物的形成机制。在本研究中，细菌纤维素（BC）纳米纤维被磷酸化，并用作磷酸钙（Ca-P）早期矿化的纳米尺寸模板。为引发矿化，将BC纳米纤维在37℃下浸入1.5倍模拟体液（1.5 SBF）中不同时间。使用扫描电子显微镜（SEM）、X射线衍射（XRD）和傅里叶变换红外光谱（FTIR）对纳米纤维表面沉积的矿物质进行表征。SEM观察证实，Ca-P矿物质的早期生长（4小时时）是不均匀的，随后矿物质在纳米纤维的整个表面广泛扩散。XRD和FTIR分析表明，磷酸八钙（OCP）是在BC纳米纤维上形成的HAp的前体。此外，沉积在BC纳米纤维上的HAp沿c轴伸长。本文分析了Ca-P矿物质的成核和生长。
