Chen Hongxia, Lee Jaebeom, Kim Sung-Hoon, Kim Jae-Ho, Koh Kwangnak
College of Nanoscience and Nanotechnology, Pusan National University, Pusan 609-735, Korea.
J Nanosci Nanotechnol. 2009 Dec;9(12):7199-203. doi: 10.1166/jnn.2009.1612.
A phenylalanine sensing system was constructed with photochromic spiroxazine derivative via surface plasmon resonance (SPR). Recognition-functional spiroxazine monolayer was formed on Au surface by self-assembly. After spectroscopic characterizations of monolayer, various concentrations of d- and l-phenylalanine were employed as analytes on UV addressable ring-opened spiroxazine monolayer. The different SPR angle shift derived from interaction between d- and l-phenylalanine and spiroxazine monolayer can be explained by the different dipole moment of ionic complexes. Computer simulations using Molecular Orbital PACkage AM1 approximation reinforced the reliability of the experimental results. To confirm the long-time stability of spiroxazine monolayer, we measured SPR response of spiroxazine monolayer with repetitive UV-on and off. These experimental results suggest that it can be applicable to simple analysis of interaction between zwitterionic recognition-functional molecule and analyte.
通过表面等离子体共振（SPR）构建了一种基于光致变色螺恶嗪衍生物的苯丙氨酸传感系统。通过自组装在金表面形成了具有识别功能的螺恶嗪单分子层。对单分子层进行光谱表征后，将不同浓度的d-和l-苯丙氨酸作为分析物应用于紫外可寻址的开环螺恶嗪单分子层上。d-和l-苯丙氨酸与螺恶嗪单分子层之间相互作用产生的不同SPR角位移可以用离子配合物不同的偶极矩来解释。使用分子轨道软件包AM1近似进行的计算机模拟增强了实验结果的可靠性。为了确认螺恶嗪单分子层的长期稳定性，我们测量了螺恶嗪单分子层在紫外光反复照射和关闭情况下的SPR响应。这些实验结果表明，它可适用于两性离子识别功能分子与分析物之间相互作用的简单分析。
