Cohn I, Heneghan J B
Department of Surgery, Louisiana State University School of Medicine, New Orleans 70112.
Am J Surg. 1991 Feb;161(2):279-83. doi: 10.1016/0002-9610(91)91145-9.
Germfree animals have been reared to a size, weight, and age permitting the performance of major surgical procedures and the pursuit of a variety of surgical research problems. Germfree dogs have been maintained in the isolator system through three generations, indicating that life, reproduction, and growth are all possible in the absence of microbial contamination. The value of the germfree approach to surgical problems has been utilized in studies of a variety of gastrointestinal problems, shock, cancer, immunology, burns, wound healing, and in direct patient application. Patients have been maintained in isolator environments for prevention of infection, for operative procedures, for treatment of extensive burns, and for management of immune-suppressed individuals. We conclude that germfree animals and germfree technics provide a valuable addition to the armamentarium of the surgeon in both research and clinical applications.
无菌动物已饲养到一定的大小、体重和年龄，能够进行大型外科手术并研究各种外科研究问题。无菌犬已在隔离器系统中维持了三代，这表明在没有微生物污染的情况下，生命、繁殖和生长都是可能的。无菌方法在解决各种胃肠道问题、休克、癌症、免疫学、烧伤、伤口愈合等外科问题的研究中，以及直接应用于患者身上都具有价值。患者已被安置在隔离环境中，用于预防感染、进行手术、治疗大面积烧伤以及管理免疫抑制个体。我们得出结论，无菌动物和无菌技术在研究和临床应用中为外科医生的装备增添了有价值的内容。
