Purdy C W, Ford S J, Grant W F
Am J Vet Res. 1977 Aug;38(8):1211-5.
An isolate of virulent equine herpesvirus (EHV) type 1 was adapted to Vero stable cell line by 13 serial passages at 37 C and 50 serial passages at 26 C. Characteristics of the attenuated EHV-1 were found to be avirulent, but immunogenic in horses if injected intramuscularly. The attenuated virus was regularly isolated from peripheral leukocytes in inoculated horses, but was not recovered from nasal turbinate tissues. A mild leukopenia was noticed. The attenuated virus produced characteristic large syncytia on primary isolation in rabbit kidney (RK13) or Vero cells at 37 C in contrast to cell rounding observed with virulent EHV-1. The syncytial marker was stable through 20 serial passages in Vero cells at 37 C. New application of double immunodiffusion test for distinguishing between EHV-1 and EHV-2 also is described.
一株强毒1型马疱疹病毒（EHV）通过在37℃下连续传代13次和在26℃下连续传代50次适应了Vero稳定细胞系。发现减毒EHV-1无毒力，但肌肉注射时在马体内具有免疫原性。在接种马的外周白细胞中经常分离到减毒病毒，但未从鼻甲组织中分离到。观察到轻度白细胞减少。与强毒EHV-1引起的细胞变圆相反，减毒病毒在37℃下初次接种于兔肾（RK13）或Vero细胞时产生特征性的大合胞体。合胞体标记在Vero细胞中于37℃连续传代20次后仍保持稳定。还描述了双免疫扩散试验用于区分EHV-1和EHV-2的新应用。
