Applied Neurotherapeutics Research Group, UCD School of Biomolecular and Biomedical Science, UCD Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland.
Brain Res Bull. 2010 Mar 16;81(4-5):385-90. doi: 10.1016/j.brainresbull.2009.11.001. Epub 2009 Nov 10.
Activity of the transcription factor NF-kappaB is required for memory formation, but the identity and function of the genes it may regulate in this context remain obscure. Here, we comprehensively characterise NF-kappaB throughout the rat hippocampus following passive avoidance training and report significant subregion-specific increased activity across the dorsoventral axis 3h post-learning. Moreover, putative NF-kappaB binding motifs predominated in structural genes previously shown to regulate 3h following avoidance conditioning, the protein products of which may be involved in the subsequent synaptic remodelling required for consolidation. Finally, we assessed the influence of NF-kappaB-mediated transcription on neuritic structure and report that inhibition of NF-kappaB significantly decreases growth and branching of primary hippocampal neurons. These results suggest that NF-kappaB activity following hippocampal learning may contribute to consolidation-associated synaptic reorganisation.
转录因子 NF-κB 的活性对于记忆形成是必需的，但它在这种情况下可能调节的基因的身份和功能仍然不清楚。在这里，我们在被动回避训练后全面描述了 NF-κB 在大鼠海马中的特征，并报告了学习后 3 小时沿背腹轴显著的亚区特异性活性增加。此外，在回避条件作用后 3 小时内被认为可以调节的结构基因中，存在 NF-κB 结合基序，其蛋白质产物可能参与随后的突触重塑，这对于巩固是必需的。最后，我们评估了 NF-κB 介导的转录对神经突结构的影响，并报告 NF-κB 的抑制显著降低了原代海马神经元的生长和分支。这些结果表明，海马学习后 NF-κB 的活性可能有助于与巩固相关的突触重组。
