Laboratoire de biologie cellulaire, UR501, IJPB, route de Saint-Cyr, INRA, 78026 Versailles, France.
C R Biol. 2009 Nov;332(11):937-46. doi: 10.1016/j.crvi.2009.09.001. Epub 2009 Oct 15.
Compartmentalization is one of the fundamental principles which underly nuclear function. Numerous studies describe complex and sometimes conflicting relationships between nuclear gene positioning and transcription regulation. Therefore the question is whether topological landmarks and/or organization principles exist to describe the nuclear architecture and, if existing, whether these principles are identical in the animal and plant kingdoms. In the frame of an agroBI-INRA program on nuclear architecture, we set up a multidisciplinary approach combining biological studies, spatial statistics and 3D modeling to investigate spatial organization of a nuclear compartment in both plant and animal cells in their physiological contexts. In this article, we review the questions addressed in this program and the methodology of our work.
区隔化是核功能的基本原则之一。许多研究描述了核基因定位和转录调控之间复杂且有时相互矛盾的关系。因此，问题在于是否存在拓扑标志和/或组织原则来描述核结构，如果存在，这些原则在动植物王国中是否相同。在一个关于核结构的 agroBI-INRA 计划框架内，我们建立了一个多学科方法，结合生物学研究、空间统计学和 3D 建模，以在生理环境中研究植物和动物细胞中核区室的空间组织。在本文中，我们回顾了该计划中所涉及的问题和我们工作的方法。
