INRA, université de Bordeaux, biologie du fruit, UMR 619, 33883 Villenave d'Ornon, France.
C R Biol. 2009 Nov;332(11):1007-21. doi: 10.1016/j.crvi.2009.09.013. Epub 2009 Oct 24.
Very few reports have studied the interactions between ascorbate and fruit metabolism. In order to get insights into the complex relationships between ascorbate biosynthesis/recycling and other metabolic pathways in the fruit, we undertook a fruit systems biology approach. To this end, we have produced tomato transgenic lines altered in ascorbate content and redox ratio by RNAi-targeting several key enzymes involved in ascorbate biosynthesis (2 enzymes) and recycling (2 enzymes). In the VTC (ViTamin C) Fruit project, we then generated phenotypic and genomic (transcriptome, proteome, metabolome) data from wild type and mutant tomato fruit at two stages of fruit development, and developed or implemented statistical and bioinformatic tools as a web application (named VTC Tool box) necessary to store, analyse and integrate experimental data in tomato. By using Kohonen's self-organizing maps (SOMs) to cluster the biological data, pair-wise Pearson correlation analyses and simultaneous visualization of transcript/protein and metabolites (MapMan), this approach allowed us to uncover major relationships between ascorbate and other metabolic pathways.
鲜有报道研究抗坏血酸与果实代谢之间的相互作用。为了深入了解果实中抗坏血酸生物合成/循环与其他代谢途径之间的复杂关系，我们采用了果实系统生物学方法。为此，我们通过 RNAi 靶向几种参与抗坏血酸生物合成（2 种酶）和循环（2 种酶）的关键酶，生成了番茄转基因株系，改变了其抗坏血酸含量和氧化还原比。在 VTC（维生素 C）果实项目中，我们从野生型和突变型番茄果实的两个发育阶段生成了表型和基因组（转录组、蛋白质组、代谢组）数据，并开发或实施了统计和生物信息学工具作为一个网络应用程序（命名为 VTC Toolbox），用于存储、分析和整合番茄中的实验数据。通过使用 Kohonen 的自组织映射图（SOM）对生物数据进行聚类，进行两两 Pearson 相关分析，并同时可视化转录本/蛋白质和代谢物（MapMan），这种方法使我们能够揭示抗坏血酸与其他代谢途径之间的主要关系。
