Niu E M, Meseguer A, Catterall J F
Population Council, Rockefeller University, New York, NY 10021.
DNA Cell Biol. 1991 Jan-Feb;10(1):41-8. doi: 10.1089/dna.1991.10.41.
The gene for kidney androgen-regulated protein (KAP) is expressed under androgenic control in the epithelial cells of the renal cortical proximal tubules. However, there is an androgen-independent component of the expression of this gene that occurs specifically in the outermedullary S3 segments of the proximal tubules. In these cells, the KAP gene is estrogen responsive and its expression is dependent on pituitary function. As a first step in correlating its interesting cell-specific and hormonal regulation with the structure of the gene, the genomic organization of the KAP gene was described and sequence of the gene and the proximal 1 kb of 5'-flanking DNA was determined. Sequence motifs were identified in the 5'-flanking DNA that may function in the regulation KAP gene expression by androgen, estrogen, and pituitary glycoprotein hormones. The gene is present in a single copy in the mouse genome and is 3,807 nucleotides in length. It contains 4 exons of 120, 177, 63, and 251 nucleotides and three intervening sequences of 1,450, 126, and 1,620 nucleotides. The gene exhibits a high degree of a genetic polymorphism as revealed by comparison of restriction digests of DNA from two highly inbred strains, BALB/c and C57BL/6.
肾雄激素调节蛋白（KAP）基因在雄激素的调控下于肾皮质近端小管的上皮细胞中表达。然而，该基因的表达存在雄激素非依赖性成分，这种成分特异性地出现在近端小管的外髓质S3段。在这些细胞中，KAP基因对雌激素有反应，其表达依赖于垂体功能。作为将其有趣的细胞特异性和激素调控与基因结构相关联的第一步，描述了KAP基因的基因组组织，并确定了该基因及其5'侧翼DNA近端1 kb的序列。在5'侧翼DNA中鉴定出了可能在雄激素、雌激素和垂体糖蛋白激素对KAP基因表达的调控中发挥作用的序列基序。该基因在小鼠基因组中以单拷贝形式存在，长度为3807个核苷酸。它包含4个外显子，分别为120、177、63和251个核苷酸，以及3个间隔序列，分别为1450、126和1620个核苷酸。通过比较两个高度近交系BALB/c和C57BL/6的DNA限制性酶切图谱发现，该基因表现出高度的遗传多态性。
